The main aim of the thesis will be to study molecular mechanisms that control the expression of transposable elements (TEs) in the human brain, with a focus on ageing, neurodegenerative disorders, and neurodevelopment. The project will use state-of-the-art molecular and cell biology approaches to examine the epigenetic status, transcriptional activity and downstream consequences of the expression of transposons in the human brain. The project will involve the use of advanced cell-culture systems such as 3D cultures (cerebral organoids) and reprogrammed induced neurons, obtained both from healthy individuals as well as patients with neurodegenerative disorders. The project will include application of different molecular biology techniques, including e.g. CUT&RUN/Tag, RNA-seq, ChIPseq, and proteomics. The thesis will also include the design and use of different CRISPR-based genetic perturbation strategies (CRISPRa, CRISPRi).