A DNA prism: Physical principles for optimizing a microfabricated DNA separation device

Lotien Richard Huang; Jonas O. Tegenfeldt; James C. Sturm; Robert H. Austin; Edward C. Cox

A DNA prism: Physical principles for optimizing a microfabricated DNA separation device

Lotien Richard Huang, Jonas O. Tegenfeldt, James C. Sturm, Robert H. Austin, Edward C. Cox

Princeton University

Forskningsoutput: Konferensbidrag › Poster › Peer review

Sammanfattning

Recently, we reported a microfabricated "DNA prism" device that continuously sorts large DNA molecules (61 kilobase pair to 209 kb) according to size in 15 seconds. In this paper, we develop models to understand and optimize the device. The device's complicated characteristics are explained poorly by a simple model based on the assumption that DNA molecules are fully stretched. Assuming DNA molecules obey Hooke's law, a second model successfully explains the "biphasic" separation characteristics under low fields. Further, the model suggests the use of high fields combined with shorter pulse durations for more linear separation dependence on molecular weight, an advantage which is then confirmed by experiments.

Originalspråk	engelska
Sidor	211-214
Antal sidor	4
Status	Published - 2002 dec. 1
Externt publicerad	Ja
Evenemang	2002 IEEE International Devices Meeting (IEDM) - San Francisco, CA, USA Varaktighet: 2002 dec. 8 → 2002 dec. 11

Konferens

Konferens	2002 IEEE International Devices Meeting (IEDM)
Land/Territorium	USA
Ort	San Francisco, CA
Period	2002/12/08 → 2002/12/11

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title = "A DNA prism: Physical principles for optimizing a microfabricated DNA separation device",

abstract = "Recently, we reported a microfabricated {"}DNA prism{"} device that continuously sorts large DNA molecules (61 kilobase pair to 209 kb) according to size in 15 seconds. In this paper, we develop models to understand and optimize the device. The device's complicated characteristics are explained poorly by a simple model based on the assumption that DNA molecules are fully stretched. Assuming DNA molecules obey Hooke's law, a second model successfully explains the {"}biphasic{"} separation characteristics under low fields. Further, the model suggests the use of high fields combined with shorter pulse durations for more linear separation dependence on molecular weight, an advantage which is then confirmed by experiments.",

author = "Huang, {Lotien Richard} and Tegenfeldt, {Jonas O.} and Sturm, {James C.} and Austin, {Robert H.} and Cox, {Edward C.}",

year = "2002",

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note = "2002 IEEE International Devices Meeting (IEDM) ; Conference date: 08-12-2002 Through 11-12-2002",

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T2 - 2002 IEEE International Devices Meeting (IEDM)

AU - Huang, Lotien Richard

AU - Tegenfeldt, Jonas O.

AU - Sturm, James C.

AU - Austin, Robert H.

AU - Cox, Edward C.

PY - 2002/12/1

Y1 - 2002/12/1

N2 - Recently, we reported a microfabricated "DNA prism" device that continuously sorts large DNA molecules (61 kilobase pair to 209 kb) according to size in 15 seconds. In this paper, we develop models to understand and optimize the device. The device's complicated characteristics are explained poorly by a simple model based on the assumption that DNA molecules are fully stretched. Assuming DNA molecules obey Hooke's law, a second model successfully explains the "biphasic" separation characteristics under low fields. Further, the model suggests the use of high fields combined with shorter pulse durations for more linear separation dependence on molecular weight, an advantage which is then confirmed by experiments.

AB - Recently, we reported a microfabricated "DNA prism" device that continuously sorts large DNA molecules (61 kilobase pair to 209 kb) according to size in 15 seconds. In this paper, we develop models to understand and optimize the device. The device's complicated characteristics are explained poorly by a simple model based on the assumption that DNA molecules are fully stretched. Assuming DNA molecules obey Hooke's law, a second model successfully explains the "biphasic" separation characteristics under low fields. Further, the model suggests the use of high fields combined with shorter pulse durations for more linear separation dependence on molecular weight, an advantage which is then confirmed by experiments.

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ER -

A DNA prism: Physical principles for optimizing a microfabricated DNA separation device

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