A global network of transcription factors, involving E2A, EBF1 and Foxo1, that orchestrates B cell fate

Yin C. Lin; Suchit Jhunjhunwala; Christopher Benner; Sven Heinz; Eva Welinder; Robert Mansson; Mikael Sigvardsson; James Hagman; Celso A. Espinoza; Janusz Dutkowski; Trey Ideker; Christopher K. Glass; Cornelis Murre

doi:10.1038/ni.1891

A global network of transcription factors, involving E2A, EBF1 and Foxo1, that orchestrates B cell fate

Yin C. Lin, Suchit Jhunjhunwala, Christopher Benner, Sven Heinz, Eva Welinder, Robert Mansson, Mikael Sigvardsson, James Hagman, Celso A. Espinoza, Janusz Dutkowski, Trey Ideker, Christopher K. Glass, Cornelis Murre

Reumatologi och molekylär skelettbiologi

Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

Sammanfattning

It is now established that the transcription factors E2A, EBF1 and Foxo1 have critical roles in B cell development. Here we show that E2A and EBF1 bound regulatory elements present in the Foxo1 locus. E2A and EBF1, as well as E2A and Foxo1, in turn, were wired together by a vast spectrum of cis-regulatory sequences. These associations were dynamic during developmental progression. Occupancy by the E2A isoform E47 directly resulted in greater abundance, as well as a pattern of monomethylation of histone H3 at lysine 4 (H3K4) across putative enhancer regions. Finally, we divided the pro-B cell epigenome into clusters of loci with occupancy by E2A, EBF and Foxo1. From this analysis we constructed a global network consisting of transcriptional regulators, signaling and survival factors that we propose orchestrates B cell fate.

Originalspråk	engelska
Sidor (från-till)	635-U109
Tidskrift	Nature Immunology
Volym	11
Nummer	7
DOI	https://doi.org/10.1038/ni.1891
Status	Published - 2010

Bibliografisk information

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Connective Tissue Biology (013230151)

Ämnesklassifikation (UKÄ)

Reumatologi och inflammation

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10.1038/ni.1891

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title = "A global network of transcription factors, involving E2A, EBF1 and Foxo1, that orchestrates B cell fate",

abstract = "It is now established that the transcription factors E2A, EBF1 and Foxo1 have critical roles in B cell development. Here we show that E2A and EBF1 bound regulatory elements present in the Foxo1 locus. E2A and EBF1, as well as E2A and Foxo1, in turn, were wired together by a vast spectrum of cis-regulatory sequences. These associations were dynamic during developmental progression. Occupancy by the E2A isoform E47 directly resulted in greater abundance, as well as a pattern of monomethylation of histone H3 at lysine 4 (H3K4) across putative enhancer regions. Finally, we divided the pro-B cell epigenome into clusters of loci with occupancy by E2A, EBF and Foxo1. From this analysis we constructed a global network consisting of transcriptional regulators, signaling and survival factors that we propose orchestrates B cell fate.",

author = "Lin, {Yin C.} and Suchit Jhunjhunwala and Christopher Benner and Sven Heinz and Eva Welinder and Robert Mansson and Mikael Sigvardsson and James Hagman and Espinoza, {Celso A.} and Janusz Dutkowski and Trey Ideker and Glass, {Christopher K.} and Cornelis Murre",

note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Connective Tissue Biology (013230151)",

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doi = "10.1038/ni.1891",

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T1 - A global network of transcription factors, involving E2A, EBF1 and Foxo1, that orchestrates B cell fate

AU - Lin, Yin C.

AU - Jhunjhunwala, Suchit

AU - Benner, Christopher

AU - Heinz, Sven

AU - Welinder, Eva

AU - Mansson, Robert

AU - Sigvardsson, Mikael

AU - Hagman, James

AU - Espinoza, Celso A.

AU - Dutkowski, Janusz

AU - Ideker, Trey

AU - Glass, Christopher K.

AU - Murre, Cornelis

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Connective Tissue Biology (013230151)

PY - 2010

Y1 - 2010

N2 - It is now established that the transcription factors E2A, EBF1 and Foxo1 have critical roles in B cell development. Here we show that E2A and EBF1 bound regulatory elements present in the Foxo1 locus. E2A and EBF1, as well as E2A and Foxo1, in turn, were wired together by a vast spectrum of cis-regulatory sequences. These associations were dynamic during developmental progression. Occupancy by the E2A isoform E47 directly resulted in greater abundance, as well as a pattern of monomethylation of histone H3 at lysine 4 (H3K4) across putative enhancer regions. Finally, we divided the pro-B cell epigenome into clusters of loci with occupancy by E2A, EBF and Foxo1. From this analysis we constructed a global network consisting of transcriptional regulators, signaling and survival factors that we propose orchestrates B cell fate.

AB - It is now established that the transcription factors E2A, EBF1 and Foxo1 have critical roles in B cell development. Here we show that E2A and EBF1 bound regulatory elements present in the Foxo1 locus. E2A and EBF1, as well as E2A and Foxo1, in turn, were wired together by a vast spectrum of cis-regulatory sequences. These associations were dynamic during developmental progression. Occupancy by the E2A isoform E47 directly resulted in greater abundance, as well as a pattern of monomethylation of histone H3 at lysine 4 (H3K4) across putative enhancer regions. Finally, we divided the pro-B cell epigenome into clusters of loci with occupancy by E2A, EBF and Foxo1. From this analysis we constructed a global network consisting of transcriptional regulators, signaling and survival factors that we propose orchestrates B cell fate.

U2 - 10.1038/ni.1891

DO - 10.1038/ni.1891

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SN - 1529-2908

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SP - 635-U109

JO - Nature Immunology

JF - Nature Immunology

IS - 7

ER -

A global network of transcription factors, involving E2A, EBF1 and Foxo1, that orchestrates B cell fate

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