A highly-contiguous genome assembly of the Eurasian spruce bark beetle, Ips typographus, provides insight into a major forest pest

Daniel Powell, Ewald Groβe-Wilde, Paal Krokene, Amit Roy, Amrita Chakraborty, Christer Löfstedt, Heiko Vogel, Martin N. Andersson, Fredrik Schlyter

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

Sammanfattning

Conifer-feeding bark beetles are important herbivores and decomposers in forest ecosystems. These species complete their life cycle in nutritionally poor substrates and some can kill enormous numbers of trees during population outbreaks. The Eurasian spruce bark beetle (Ips typographus) can destroy >100 million m3 of spruce in a single year. We report a 236.8 Mb I. typographus genome assembly using PacBio long-read sequencing. The final phased assembly has a contig N50 of 6.65 Mb in 272 contigs and is predicted to contain 23,923 protein-coding genes. We reveal expanded gene families associated with plant cell wall degradation, including pectinases, aspartyl proteases, and glycosyl hydrolases. This genome sequence from the genus Ips provides timely resources to address questions about the evolutionary biology of the true weevils (Curculionidae), one of the most species-rich animal families. In forests of today, increasingly stressed by global warming, this draft genome may assist in developing pest control strategies to mitigate outbreaks.

Originalspråkengelska
Artikelnummer1059
Antal sidor9
TidskriftCommunications Biology
Volym4
DOI
StatusPublished - 2021 sep 9

Bibliografisk information

Funding Information:
We would like to acknowledge the support of the National Genomics Infrastructure (NGI)/ Uppsala Genome Center and UPPMAX for providing assistance in massive parallel sequencing and computational infrastructure. Work performed at NGI / Uppsala Genome Center has been funded by RFI/VR and Science for Life Laboratory, Sweden. We thank several colleagues for discussions and help on genome sizes, chromosomes, and long contigs data: J. Spencer Johnston (Texas A&M University, College Station), Anthony I. Cognato (Michigan State University, East Lansing), and Krystyna Nadachowska-Brzyska & Piotr Zieliński (Jagiellonian University, Krakow). Dr Jan Bílý (Czech University of Life Sciences, Prague) is acknowledged for technical support during RNA samples preparation for tissue transcriptome study. Infrastructural support and salary for A.R., E.G-W. and F.S. were obtained from project EXTEMIT-K CZ.02.1.01/0.0/0.0/15_003/0000433 financed by OP RDE at Czech University of Life Sciences, Prague. M.N.A. was funded by the Swedish Research Council FORMAS (grants #217-2014-689 and #2018-01444). C.L. acknowledges support from the Swedish Research Council VR (grant #2017-03804). P.K. was funded by the Research Council of Norway (grant #249958/F20).

Publisher Copyright:
© 2021, The Author(s).

Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.

Ämnesklassifikation (UKÄ)

  • Biokemi och molekylärbiologi
  • Evolutionsbiologi
  • Ekologi

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