A multiscale analysis of early flower development in Arabidopsis provides an integrated view of molecular regulation and growth control

Yassin Refahi, Argyris Zardilis, Gaël Michelin, Raymond Wightman, Bruno Leggio, Jonathan Legrand, Emmanuel Faure, Laetitia Vachez, Alessia Armezzani, Anne Evodie Risson, Feng Zhao, Pradeep Das, Nathanaël Prunet, Elliot M. Meyerowitz, Christophe Godin, Grégoire Malandain, Henrik Jönsson, Jan Traas

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

3 Citeringar (Scopus)

Sammanfattning

We have analyzed the link between the gene regulation and growth during the early stages of flower development in Arabidopsis. Starting from time-lapse images, we generated a 4D atlas of early flower development, including cell lineage, cellular growth rates, and the expression patterns of regulatory genes. This information was introduced in MorphoNet, a web-based platform. Using computational models, we found that the literature-based molecular network only explained a minority of the gene expression patterns. This was substantially improved by adding regulatory hypotheses for individual genes. Correlating growth with the combinatorial expression of multiple regulators led to a set of hypotheses for the action of individual genes in morphogenesis. This identified the central factor LEAFY as a potential regulator of heterogeneous growth, which was supported by quantifying growth patterns in a leafy mutant. By providing an integrated view, this atlas should represent a fundamental step toward mechanistic models of flower development. Refahi and Zardilis et al. present a 4D atlas of early flower development. They combine growth and gene expression to construct a molecular network model that correctly predicts a large majority of 28 gene expression patterns. This model suggests hypotheses for the combined action of regulatory genes in morphogenesis.

Originalspråkengelska
Sidor (från-till)540-556.e8
TidskriftDevelopmental Cell
Volym56
Utgåva4
DOI
StatusPublished - 2021

Ämnesklassifikation (UKÄ)

  • Botanik
  • Genetik

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