A simple assay for the detection of antibodies to endocrine islet cell surface antigens

Giovanna Contreas, Ole D. Madsen, Henrik Vissing, Åke Lernmark

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

Sammanfattning

A simple and sensitive immunoradiometric assay for the detection of islet cell surface antibodies (CIRMA) has been developed. Live, transformed islet cells derived from a liver metastasis of a transplantable islet cell tumor were grown in removable microtiter wells and incubated with antibody. Cell-bound antibodies were quantitated using 125I-labelled second antibodies. The assay was used to detect islet cell antibodies present in sera from non-diabetic and diabetic BB rats and proved to be particularly effective for screening hybridoma supernatants in order to identify monoclonal antibodies recognizing islet cell surface antigens.

Originalspråkengelska
Sidor (från-till)135-139
Antal sidor5
TidskriftJournal of Immunological Methods
Volym95
Nummer1
DOI
StatusPublished - 1986 dec. 4
Externt publiceradJa

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