Acoustic Differential Extraction - ultrasonic DNA-extraction from sexual assault evidence

Mikael Evander, Katie M. Horsman, Christopher J. Easley, James P. Landers, Thomas Laurell, Johan Nilsson

Forskningsoutput: Kapitel i bok/rapport/Conference proceedingKonferenspaper i proceedingPeer review

Sammanfattning

Isolation of the male and female DNA is one of the most important steps in obtaining the DNA profile of the perpetrator in sexual assault cases. The sample is obtained by taking a vaginal swab containing both epithelial cells from the female and sperm cells from the male from the victim. The purity of the extracted male fraction decides whether or not a single-source DNA profile of the suspect will be obtained or not. The existing techniques are have poor separation efficiency, time-consuming, labour-intensive and are neither easily automated nor integrated with further analysis steps.
A novel method of DNA extraction based on ultrasonic trapping, Acoustic Differential Extraction, has been developed. A microfluidic device using laminar flow valving and miniature PZT transducers retains the sperm cells while mobilizing the female fraction into a separate outlet. The device was evaluated using a mock sexual assault sample and the separated fractions were analyzed using quantitative PCR and STR-profiling.
A 16-fold enrichment of the male fraction, making an originally hard-to-detect-male DNA profile readily profiled, has been demonstrated. The STR-profiling of the male and female fractions showed a male fraction purity of up to 99 % making it possible to obtain a single-source DNA-profile of the suspect. The microfluidic format of the device makes it possible to downscale the sample time from 4-8 hours to 10 minutes. It is also possible to integrate this method with further downstream analysis steps necessarey for the full forensic DNA analysis.
Originalspråkengelska
Titel på värdpublikationInternational Congress on Ultrasonics
RedaktörerEwald Benes
FörlagInternational Congress on Ultrasonics
Sidor151-151
Antal sidor1
Volym1
StatusPublished - 2007
EvenemangInternational Congress on Ultrasonics 2007 - Wien
Varaktighet: 2007 apr. 9 → …

Publikationsserier

Namn
Volym1

Konferens

KonferensInternational Congress on Ultrasonics 2007
Period2007/04/09 → …

Ämnesklassifikation (UKÄ)

  • Medicinteknik

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