Active and separate secretion of fiber and penton base during the early phase of Ad2 or Ad5 infection

Yuhua Yan, Bo Zhang, Weihong Hou, Hongyu Lin, Johan Rebetz, Saw See Hong, Youjun Wang, Liang Ran, Xiaolong Fan

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

Sammanfattning

Fiber and penton base overproduced in adenovirus (Ad) infected cells can be secreted prior to progeny release and thereby regulate progeny spread. We aimed to investigate the mechanisms of fiber and penton base secretion in Ad2- or Ad5-infected A549 cells. Our flow cytometry analyses detected abundant surface fiber molecules, but little penton base molecules at 12 h post infection. Immunogold staining combined with transmission electron microscopic analyses revealed separate, non-co-localized release of fiber and penton base in the proximity of the plasma membrane. Depolymerization of microtubule and actin cytoskeletons, and inhibition of Rock kinase and myosin II activity together demonstrated cytoskeletal network-dependent fiber secretion. Inhibition of intracellular calcium [Ca2+]i signaling caused diminished fiber secretion, which was associated with diminished progeny production. Thus, fiber and penton base are actively and separately secreted during the early stages of Ad2 or Ad5 infection, their secretion may play important role in Ad life cycle.

Originalspråkengelska
Sidor (från-till)172-180
Antal sidor9
TidskriftVirology
Volym505
DOI
StatusPublished - 2017 maj 1

Ämnesklassifikation (UKÄ)

  • Mikrobiologi inom det medicinska området

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