Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models.

Mehdi Djelloul; Staffan Holmqvist; Antonio Boza Serrano; Carla Azevedo; Maggie S Yeung; Stefano Goldwurm; Jonas Frisén; Tomas Deierborg; Laurent Roybon

doi:10.1016/j.stemcr.2015.07.002

Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models.

Mehdi Djelloul, Staffan Holmqvist, Antonio Boza Serrano, Carla Azevedo, Maggie S Yeung, Stefano Goldwurm, Jonas Frisén, Tomas Deierborg, Laurent Roybon

Karolinska Institutet

Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

394 Nedladdningar (Pure)

Sammanfattning

In this study, we sought evidence for alpha-synuclein (ASYN) expression in oligodendrocytes, as a possible endogenous source of ASYN to explain its presence in glial inclusions found in multiple system atrophy (MSA) and Parkinson's disease (PD). We identified ASYN in oligodendrocyte lineage progenitors isolated from the rodent brain, in oligodendrocytes generated from embryonic stem cells, and in induced pluripotent stem cells produced from fibroblasts of a healthy individual and patients diagnosed with MSA or PD, in cultures in vitro. Notably, we observed a significant decrease in ΑSYN during oligodendrocyte maturation. Additionally, we show the presence of transcripts in PDGFRΑ/CD140a(+) cells and SOX10(+) oligodendrocyte lineage nuclei isolated by FACS from rodent and human healthy and diseased brains, respectively. Our work identifies ASYN in oligodendrocyte lineage cells, and it offers additional in vitro cellular models that should provide significant insights of the functional implication of ASYN during oligodendrocyte development and disease.

Originalspråk	engelska
Sidor (från-till)	174-184
Tidskrift	Stem Cell Reports
Volym	5
Nummer	2
DOI	https://doi.org/10.1016/j.stemcr.2015.07.002
Status	Published - 2015

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Cell- och molekylärbiologi

FN:s Globala mål

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10.1016/j.stemcr.2015.07.002

Cell_press_Djelloul

http://www.ncbi.nlm.nih.gov/pubmed/26235891?dopt=Abstract

2 Doktorsavhandling (sammanläggning)

Oligodendroglial (dys)function in alpha-synucleinopathies
Azevedo, C., 2020, Lund: Lund University, Faculty of Medicine. 119 s.
Forskningsoutput: Avhandling › Doktorsavhandling (sammanläggning)

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Generating new models to study propagation and pathology of alpha-synuclein in Parkinson's disease and multiple system atrophy
Holmqvist, S., 2016, Lund: Lund University: Faculty of Medicine. 94 s.
Forskningsoutput: Avhandling › Doktorsavhandling (sammanläggning)

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title = "Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models.",

abstract = "In this study, we sought evidence for alpha-synuclein (ASYN) expression in oligodendrocytes, as a possible endogenous source of ASYN to explain its presence in glial inclusions found in multiple system atrophy (MSA) and Parkinson's disease (PD). We identified ASYN in oligodendrocyte lineage progenitors isolated from the rodent brain, in oligodendrocytes generated from embryonic stem cells, and in induced pluripotent stem cells produced from fibroblasts of a healthy individual and patients diagnosed with MSA or PD, in cultures in vitro. Notably, we observed a significant decrease in ΑSYN during oligodendrocyte maturation. Additionally, we show the presence of transcripts in PDGFRΑ/CD140a(+) cells and SOX10(+) oligodendrocyte lineage nuclei isolated by FACS from rodent and human healthy and diseased brains, respectively. Our work identifies ASYN in oligodendrocyte lineage cells, and it offers additional in vitro cellular models that should provide significant insights of the functional implication of ASYN during oligodendrocyte development and disease.",

author = "Mehdi Djelloul and Staffan Holmqvist and {Boza Serrano}, Antonio and Carla Azevedo and Yeung, {Maggie S} and Stefano Goldwurm and Jonas Fris{\'e}n and Tomas Deierborg and Laurent Roybon",

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doi = "10.1016/j.stemcr.2015.07.002",

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AU - Djelloul, Mehdi

AU - Holmqvist, Staffan

AU - Boza Serrano, Antonio

AU - Azevedo, Carla

AU - Yeung, Maggie S

AU - Goldwurm, Stefano

AU - Frisén, Jonas

AU - Deierborg, Tomas

AU - Roybon, Laurent

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N2 - In this study, we sought evidence for alpha-synuclein (ASYN) expression in oligodendrocytes, as a possible endogenous source of ASYN to explain its presence in glial inclusions found in multiple system atrophy (MSA) and Parkinson's disease (PD). We identified ASYN in oligodendrocyte lineage progenitors isolated from the rodent brain, in oligodendrocytes generated from embryonic stem cells, and in induced pluripotent stem cells produced from fibroblasts of a healthy individual and patients diagnosed with MSA or PD, in cultures in vitro. Notably, we observed a significant decrease in ΑSYN during oligodendrocyte maturation. Additionally, we show the presence of transcripts in PDGFRΑ/CD140a(+) cells and SOX10(+) oligodendrocyte lineage nuclei isolated by FACS from rodent and human healthy and diseased brains, respectively. Our work identifies ASYN in oligodendrocyte lineage cells, and it offers additional in vitro cellular models that should provide significant insights of the functional implication of ASYN during oligodendrocyte development and disease.

AB - In this study, we sought evidence for alpha-synuclein (ASYN) expression in oligodendrocytes, as a possible endogenous source of ASYN to explain its presence in glial inclusions found in multiple system atrophy (MSA) and Parkinson's disease (PD). We identified ASYN in oligodendrocyte lineage progenitors isolated from the rodent brain, in oligodendrocytes generated from embryonic stem cells, and in induced pluripotent stem cells produced from fibroblasts of a healthy individual and patients diagnosed with MSA or PD, in cultures in vitro. Notably, we observed a significant decrease in ΑSYN during oligodendrocyte maturation. Additionally, we show the presence of transcripts in PDGFRΑ/CD140a(+) cells and SOX10(+) oligodendrocyte lineage nuclei isolated by FACS from rodent and human healthy and diseased brains, respectively. Our work identifies ASYN in oligodendrocyte lineage cells, and it offers additional in vitro cellular models that should provide significant insights of the functional implication of ASYN during oligodendrocyte development and disease.

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DO - 10.1016/j.stemcr.2015.07.002

M3 - Article

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JO - Stem Cell Reports

JF - Stem Cell Reports

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Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models.

Sammanfattning

Ämnesklassifikation (UKÄ)

FN:s Globala mål

Tillgång till dokument

Fingeravtryck

Forskningsoutput

Oligodendroglial (dys)function in alpha-synucleinopathies

Generating new models to study propagation and pathology of alpha-synuclein in Parkinson's disease and multiple system atrophy

Citera det här