Amyloid-like amelogenin nanoribbons template mineralization via a low-energy interface of ion binding sites

Susrut Akkineni, Cheng Zhu, Jiajun Chen, Miao Song, Samuel E. Hoff, Johan Bonde, Jinhui Tao, Hendrik Heinz, Stefan Habelitz, James J. De Yoreo

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

Sammanfattning

Protein scaffolds direct the organization of amorphous precursors that transform into mineralized tissues, but the templating mechanism remains elusive. Motivated by models for the biomineralization of tooth enamel, wherein amyloid-like amelogenin nanoribbons guide the mineralization of apatite filaments, we investigated the impact of nanoribbon structure, sequence, and chemistry on amorphous calcium phosphate (ACP) nucleation. Using full-length human amelogenin and peptide analogs with an amyloid-like domain, films of β-sheet nanoribbons were self-assembled on graphite and characterized by in situ atomic force microscopy and molecular dynamics simulations. All sequences substantially reduce nucleation barriers for ACP by creating low-energy interfaces, while phosphoserines along the length of the nanoribbons dramatically enhance kinetic factors associated with ion binding. Furthermore, the distribution of negatively charged residues along the nanoribbons presents a potential match to the Ca–Ca distances of the multi-ion complexes that constitute ACP. These findings show that amyloid-like amelogenin nanoribbons provide potent scaffolds for ACP mineralization by presenting energetically and stereochemically favorable templates of calcium phosphate ion binding and suggest enhanced surface wetting toward calcium phosphates in general.
Originalspråkengelska
Artikelnummere2106965119
Antal sidor10
TidskriftProceedings of the National Academy of Sciences of the United States of America
Volym119
Nummer19
DOI
StatusPublished - 2022 maj 10

Ämnesklassifikation (UKÄ)

  • Biokemi och molekylärbiologi
  • Odontologi

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