An AC Electrokinetic Device for the rapid separation and detection of cancer related DNA nanoparticulate biomarkers

Rajaram Krishnan, David Charlot, Lucas Kumosa, William Hanna, Jerry Lu, Avery Sonnenberg, Michael Heller

Forskningsoutput: Kapitel i bok/rapport/Conference proceedingKonferenspaper i proceedingPeer review

Sammanfattning

The ability to rapidly detect cell free circulating (cfc) DNA and other nanoparticulate disease biomarkers directly in blood is a major challenge for nanomedicine. We now show that AC Electrokinetic (ACE) microelectrode array devices can be used to rapidly isolate and detect cfc-DNA nanoparticulates directly from whole blood and other high conductance samples (plasma, serum etc.). The device shows isolation of naked DNA in water and plasma at >500bp size and can isolate DNA down to 50 picograms in 50uL of fluid. Disease specific cfc-DNA materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia.

Originalspråkengelska
Titel på värdpublikationBiomedical Circuits and Systems Conference (BioCAS), 2011 IEEE
UtgivningsortSan Diego
Sidor373-376
DOI
StatusPublished - 2011
Externt publiceradJa
Evenemang2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011 - San Diego, CA, USA
Varaktighet: 2011 nov. 102011 nov. 12

Konferens

Konferens2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011
Land/TerritoriumUSA
OrtSan Diego, CA
Period2011/11/102011/11/12

Ämnesklassifikation (UKÄ)

  • Biomedicinsk laboratorievetenskap/teknologi

Fingeravtryck

Utforska forskningsämnen för ”An AC Electrokinetic Device for the rapid separation and detection of cancer related DNA nanoparticulate biomarkers”. Tillsammans bildar de ett unikt fingeravtryck.

Citera det här