TY - JOUR
T1 - An ELISA for hCAP-18, the cathelicidin present in human neutrophils and plasma
AU - Sørensen, O
AU - Cowland, J B
AU - Askaa, J
AU - Borregaard, N
PY - 1997/8/7
Y1 - 1997/8/7
N2 - hCAP-18 is a newly described protein of human neutrophilic granulocytes which belongs to the cathelicidin family of antimicrobial proteins. Members of this protein family share a common N-terminal sequence followed by a highly diverse antimicrobial, cationic C-terminus. The present work describes the production of recombinant hCAP-18, the generation of antibodies to the protein and the development of an accurate, sensitive and specific ELISA for the detection of hCAP-18 in cells, plasma and urine with a detection limit of 0.084 ng/ml. The amount of hCAP-18 in neutrophils is 0.627 microgram protein per 10(6) cells. The plasma level is 1.18 micrograms/ml which is several fold higher than for other neutrophil specific granule proteins. hCAP-18 is present in plasma as high molecular weight complexes. In accordance with this, hCAP-18 is barely excreted in the urine. The bone marrow appears to be the major source of plasma hCAP-18. The high level of hCAP-18 in plasma may provide an important defense against microorganisms and endotoxins.
AB - hCAP-18 is a newly described protein of human neutrophilic granulocytes which belongs to the cathelicidin family of antimicrobial proteins. Members of this protein family share a common N-terminal sequence followed by a highly diverse antimicrobial, cationic C-terminus. The present work describes the production of recombinant hCAP-18, the generation of antibodies to the protein and the development of an accurate, sensitive and specific ELISA for the detection of hCAP-18 in cells, plasma and urine with a detection limit of 0.084 ng/ml. The amount of hCAP-18 in neutrophils is 0.627 microgram protein per 10(6) cells. The plasma level is 1.18 micrograms/ml which is several fold higher than for other neutrophil specific granule proteins. hCAP-18 is present in plasma as high molecular weight complexes. In accordance with this, hCAP-18 is barely excreted in the urine. The bone marrow appears to be the major source of plasma hCAP-18. The high level of hCAP-18 in plasma may provide an important defense against microorganisms and endotoxins.
KW - Animals
KW - Anti-Infective Agents/blood
KW - Antibodies/chemistry
KW - Antimicrobial Cationic Peptides
KW - Carrier Proteins/blood
KW - Cathelicidins
KW - Enzyme-Linked Immunosorbent Assay/methods
KW - Humans
KW - Neutrophils/chemistry
KW - Plasma/chemistry
KW - Rabbits
KW - Reproducibility of Results
KW - Sensitivity and Specificity
U2 - 10.1016/s0022-1759(97)00084-7
DO - 10.1016/s0022-1759(97)00084-7
M3 - Article
C2 - 9328568
SN - 0022-1759
VL - 206
SP - 53
EP - 59
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1-2
ER -