TY - JOUR
T1 - An Improvement on MS-based Epigenetic Analysis of Large Histone-derived Peptides by Using the IVU Interface.
AU - Fukuda, Tetsuya
AU - Hike, Hiroshi
AU - Usui, Fumihiko
AU - Bando, Yasuhiko
AU - Nishimura, Toshide
AU - Kodama, Tatsuhiko
AU - Kawamura, Takeshi
PY - 2015
Y1 - 2015
N2 - Highly protonated histone-derived peptides impede a sufficient mass spectrometry (MS) -based epigenetic analysis since their relatively low m/z due to a high degree of proton addition to peptides would difficult to analyze the resulting complex MS/MS spectra. In order to reduce the degree of protonations, we have developed a new interface, the IVU, in which peptides are ionized under a vaporized organic solvent, and it has demonstrated that the doubly-charged histone-tail H2B peptide, PEPAKSAPAPKKGSKKAVTKAQKK (m/z 1238.243 , +2), has been detected by using the IVU interface and sequenced, which was not detectable before.
AB - Highly protonated histone-derived peptides impede a sufficient mass spectrometry (MS) -based epigenetic analysis since their relatively low m/z due to a high degree of proton addition to peptides would difficult to analyze the resulting complex MS/MS spectra. In order to reduce the degree of protonations, we have developed a new interface, the IVU, in which peptides are ionized under a vaporized organic solvent, and it has demonstrated that the doubly-charged histone-tail H2B peptide, PEPAKSAPAPKKGSKKAVTKAQKK (m/z 1238.243 , +2), has been detected by using the IVU interface and sequenced, which was not detectable before.
U2 - 10.1016/j.ab.2015.06.002
DO - 10.1016/j.ab.2015.06.002
M3 - Article
C2 - 26050628
SN - 1096-0309
VL - 486
SP - 14
EP - 16
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - Online 05 June 2015
ER -