Sammanfattning
The invention discloses an optical circulation enlarged type biological chip detecting method, wherein the enzyme recirculation and bioluminescence are combined, for realizing the FMNH#-[2] accumulation through the circulation on the electrodes by the NADH/NAD#+[+] oxidation-reduction, the high level accumulation FMNH#-[2] reacts with other light-emitting bottom luciferase, long chain aldehydes and oxygen, giving off fluorescent light having wavelength of 490nm. The invention realizes high sensibility, low cost and simplicity of operation, thus is suitable for each types of biological sample analysis.
| Bidragets översatta titel | An optical circulation amplification type biological chip detecting process |
|---|---|
| Originalspråk | kinesiska (förenklad) |
| Patentnummer | CN1485617A |
| IPC | C12Q1/25,G01N33/50,G01N33/576,(IPC1-7): C12Q1/25,G01N33/50,G01N33/576 |
| Prioritetsdatum | 2002/09/26 |
| Arkiveringsdatum | 2002/09/26 |
| Status | Published - 2004 |
Ämnesklassifikation (UKÄ)
- Biologi
- Analytisk kemi