Analyses of the complex formation of staphylococcal enterotoxin A and the human gp130 cytokine receptor

Sibel Uzuncayir; Arturo  Vera-Rodriguez; Paulina Regenthal; Hannah Åbacka; Cecilia Emanuelsson; Christopher D. Bahl; Karin Lindkvist-Petersson

doi:10.1002/1873-3468.14292

Analyses of the complex formation of staphylococcal enterotoxin A and the human gp130 cytokine receptor

Sibel Uzuncayir, Arturo Vera-Rodriguez, Paulina Regenthal, Hannah Åbacka, Cecilia Emanuelsson, Christopher D. Bahl, Karin Lindkvist-Petersson

Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

Sammanfattning

Superantigens (SAgs) are bacterial enterotoxins produced by Staphylococcus aureus. Staphylococcal enterotoxin type A (SEA), a staphylococcal superantigen, has been shown to bind to the cytokine signalling receptor glycoprotein 130 (gp130). The structural details, as well as the exact physiological role of this interaction, remain unclear. Here, we describe the structural details of the SEA–gp130 complex by combining crosslinking mass spectrometry and computational modelling. Interestingly, SEA is not able to bind gp130-homologues from rat and mouse. Our data suggest that SEA may interact with human gp130 in a different manner than other known gp130-ligands. Moreover, the fact that SEA does not bind mouse or rat gp130 suggests that SAgs have additional mechanisms of action in humans.

Originalspråk	engelska
Sidor (från-till)	910-923
Tidskrift	FEBS Letters
Volym	596
Nummer	7
Tidigt onlinedatum	2022
DOI	https://doi.org/10.1002/1873-3468.14292
Status	Published - 2022

Ämnesklassifikation (UKÄ)

Immunologi inom det medicinska området
Mikrobiologi inom det medicinska området

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10.1002/1873-3468.14292Licens: CC BY

1 Doktorsavhandling (sammanläggning)

Dangerous Proteins and Where to Find Them- Structural and functional studies of bacterial and viral proteins interacting with human immune receptors in health and disease
Uzuncayir, S., 2022, Lund: Lund University, Faculty of Medicine. 77 s.
Forskningsoutput: Avhandling › Doktorsavhandling (sammanläggning)

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title = "Analyses of the complex formation of staphylococcal enterotoxin A and the human gp130 cytokine receptor",

abstract = "Superantigens (SAgs) are bacterial enterotoxins produced by Staphylococcus aureus. Staphylococcal enterotoxin type A (SEA), a staphylococcal superantigen, has been shown to bind to the cytokine signalling receptor glycoprotein 130 (gp130). The structural details, as well as the exact physiological role of this interaction, remain unclear. Here, we describe the structural details of the SEA–gp130 complex by combining crosslinking mass spectrometry and computational modelling. Interestingly, SEA is not able to bind gp130-homologues from rat and mouse. Our data suggest that SEA may interact with human gp130 in a different manner than other known gp130-ligands. Moreover, the fact that SEA does not bind mouse or rat gp130 suggests that SAgs have additional mechanisms of action in humans.",

author = "Sibel Uzuncayir and Arturo Vera-Rodriguez and Paulina Regenthal and Hannah {\AA}backa and Cecilia Emanuelsson and Bahl, {Christopher D.} and Karin Lindkvist-Petersson",

year = "2022",

doi = "10.1002/1873-3468.14292",

language = "English",

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T1 - Analyses of the complex formation of staphylococcal enterotoxin A and the human gp130 cytokine receptor

AU - Uzuncayir, Sibel

AU - Vera-Rodriguez, Arturo

AU - Regenthal, Paulina

AU - Åbacka, Hannah

AU - Emanuelsson, Cecilia

AU - Bahl, Christopher D.

AU - Lindkvist-Petersson, Karin

PY - 2022

Y1 - 2022

N2 - Superantigens (SAgs) are bacterial enterotoxins produced by Staphylococcus aureus. Staphylococcal enterotoxin type A (SEA), a staphylococcal superantigen, has been shown to bind to the cytokine signalling receptor glycoprotein 130 (gp130). The structural details, as well as the exact physiological role of this interaction, remain unclear. Here, we describe the structural details of the SEA–gp130 complex by combining crosslinking mass spectrometry and computational modelling. Interestingly, SEA is not able to bind gp130-homologues from rat and mouse. Our data suggest that SEA may interact with human gp130 in a different manner than other known gp130-ligands. Moreover, the fact that SEA does not bind mouse or rat gp130 suggests that SAgs have additional mechanisms of action in humans.

AB - Superantigens (SAgs) are bacterial enterotoxins produced by Staphylococcus aureus. Staphylococcal enterotoxin type A (SEA), a staphylococcal superantigen, has been shown to bind to the cytokine signalling receptor glycoprotein 130 (gp130). The structural details, as well as the exact physiological role of this interaction, remain unclear. Here, we describe the structural details of the SEA–gp130 complex by combining crosslinking mass spectrometry and computational modelling. Interestingly, SEA is not able to bind gp130-homologues from rat and mouse. Our data suggest that SEA may interact with human gp130 in a different manner than other known gp130-ligands. Moreover, the fact that SEA does not bind mouse or rat gp130 suggests that SAgs have additional mechanisms of action in humans.

U2 - 10.1002/1873-3468.14292

DO - 10.1002/1873-3468.14292

M3 - Article

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VL - 596

SP - 910

EP - 923

JO - FEBS Letters

JF - FEBS Letters

SN - 1873-3468

IS - 7

ER -

Analyses of the complex formation of staphylococcal enterotoxin A and the human gp130 cytokine receptor

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Ämnesklassifikation (UKÄ)

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Forskningsoutput

Dangerous Proteins and Where to Find Them- Structural and functional studies of bacterial and viral proteins interacting with human immune receptors in health and disease

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