Analysis of the immunochemical relationship between antigens by electrophoresis in agarose gels containing antibodies

A procedure is described, in which two antigens are brought to interact during their electrophoretic migration in an agarose gel containing antiserum. Three fundamental precipitation patterns can be discerned. These patterns correspond to the three basic precipitation patterns obtained with the double radial immunodiffusion procedure of Ouchterlony. The procedure described here can thus be used in the analysis of the immunochemical relationsship between antigens. It may also be used for estimating the percentage of cross-reacting antibodies in an antiserum. The procedure requires only a few hours.