Bet v 1 homologues in strawberry identified as IgE-binding proteins and presumptive allergens

Anne-Li Karlsson, Rikard Alm, Bo Ekstrand, Stina Fjelkner-Modig, Åsa Schiött, Ulf Bengtsson, Lars Björk, Karin Hjerno, Peter Roepstorff, Cecilia Emanuelsson

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

268 Nedladdningar (Pure)

Sammanfattning

Background: No strawberry allergen has so far been identified and characterized.
Methods: Serum samples were collected from patients with a suggestive case history of adverse reactions to strawberry and other fruits. Extracts from fresh
and frozen strawberries were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting and mass spectrometry. Patient blood samples were analysed for inhibition of IgE binding and basophil degranulation.
Results: Several IgE-binding proteins could be detected. In more than half of the patient sera, a 20/18-kDa doublet band was observed in Western blotting. These
two bands were excised and analysed by mass spectrometry showing the presence of proteins belonging to the Bet v 1 family of allergens. Inhibition of the IgE binding to the 20/18-kDa doublet was obtained by addition of two recombinantly expressed allergens belonging to the Bet v 1 family (Bet v 1 and Mal d 1) and strawberry protein extract. In a cell-based assay of patient blood samples,
basophil degranulation could be induced by strawberry protein extract and by Bet v 1 and Mal d 1.
Conclusions: We conclude that strawberry homologues to Bet v 1 may be allergens of importance for adverse reactions to strawberry.
Originalspråkengelska
Sidor (från-till)1277-1284
TidskriftAllergy
Volym59
Nummer12
DOI
StatusPublished - 2004

Ämnesklassifikation (UKÄ)

  • Lungmedicin och allergi

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