Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time

Gizem Ertürk Bergdahl, Martin Hedström, Bo Mattiasson

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

1 Citering (SciVal)

Sammanfattning

A capacitive sensor was developed to analyze the presence and enzymatic activity of a model protease from standard solutions by following the degradation of the substrate in real time. The enzyme was chosen based on its specific digestion of the hinge region of immunoglobulin G (IgG). Real-time enzyme activity was monitored by measuring the change in capacitance (∆C) based on the release of IgG fragments after enzymatic digestion by the enzyme. The results indicated that the developed capacitive system might be used successfully for label-free and real-time monitoring of enzymatic activity of different enzymes in a sensitive, rapid, and inexpensive manner in biotechnological, environmental, and clinical applications.

Originalspråkengelska
Sidor (från-till)374–383
Antal sidor10
TidskriftApplied Biochemistry and Biotechnology
Volym189
Tidigt onlinedatum2019
DOI
StatusPublished - 2019 okt

Ämnesklassifikation (UKÄ)

  • Biomedicinsk laboratorievetenskap/teknologi
  • Medicinsk laboratorie- och mätteknik

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