Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy

Gustavo de Medeiros, Dimitri Kromm, Balint Balazs, Nils Norlin, Stefan Günther, Emiliano Izquierdo, Paolo Ronchi, Shinya Komoto, Uros Krzic, Yannick Schwab, Francesca Peri, Stefano de Renzis, Maria Leptin, Matteo Rauzi, Lars Hufnagel

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

7 Citeringar (SciVal)

Sammanfattning

Three-dimensional live imaging has become an indispensable technique in the fields of cell, developmental and neural biology. Precise spatio-temporal manipulation of biological entities is often required for a deeper functional understanding of the underlying biological process. Here we present a home-built integrated framework and optical design that combines three-dimensional light-sheet imaging over time with precise spatio-temporal optical manipulations induced by short infrared laser pulses. We demonstrate their potential for sub-cellular ablation of neurons and nuclei, tissue cauterization and optogenetics by using the Drosophila melanogaster and zebrafish model systems.

Originalspråkengelska
Sidor (från-till)1942
TidskriftScientific Reports
Volym10
Utgåva1
DOI
StatusPublished - 2020 feb 6
Externt publiceradJa

Ämnesklassifikation (UKÄ)

  • Cell- och molekylärbiologi

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