Cellobiose dehydrogenase modified electrodes: advances by materials science and biochemical engineering

Roland Ludwig, Roberto Ortiz, Christopher Schulz, Wolfgang Harreither, Christoph Sygmund, Lo Gorton

Forskningsoutput: TidskriftsbidragÖversiktsartikelPeer review

102 Citeringar (SciVal)

Sammanfattning

The flavocytochrome cellobiose dehydrogenase (CDH) is a versatile biorecognition element capable of detecting carbohydrates as well as quinones and catecholamines. In addition, it can be used as an anode biocatalyst for enzymatic biofuel cells to power miniaturised sensor-transmitter systems. Various electrode materials and designs have been tested in the past decade to utilize and enhance the direct electron transfer (DET) from the enzyme to the electrode. Additionally, mediated electron transfer (MET) approaches via soluble redox mediators and redox polymers have been pursued. Biosensors for cellobiose, lactose and glucose determination are based on CDH from different fungal producers, which show differences with respect to substrate specificity, pH optima, DET efficiency and surface binding affinity. Biosensors for the detection of quinones and catecholamines can use carbohydrates for analyte regeneration and signal amplification. This review discusses different approaches to enhance the sensitivity and selectivity of CDH-based biosensors, which focus on (1) more efficient DET on chemically modified or nanostructured electrodes, (2) the synthesis of custom-made redox polymers for higher MET currents and (3) the engineering of enzymes and reaction pathways. Combination of these strategies will enable the design of sensitive and selective CDH-based biosensors with reduced electrode size for the detection of analytes in continuous on-site and point-of-care applications.
Originalspråkengelska
Sidor (från-till)3637-3658
TidskriftAnalytical and Bioanalytical Chemistry
Volym405
Nummer11
DOI
StatusPublished - 2013

Bibliografisk information

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Biochemistry and Structural Biology (S) (000006142), Analytical Chemistry (S/LTH) (011001004)

Ämnesklassifikation (UKÄ)

  • Analytisk kemi

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