Cellular nutrient content measured with the nuclear microprobe and toxins produced by Dinophysis norvegica (Dinophyceae) from the Trondheim fjord (Norway)

Eva Perez Blanco, Chatarina Karlsson, Jan Pallon, Takeshi Yasumoto, Edna Graneli

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

2 Citeringar (SciVal)

Sammanfattning

Dinophysis norvegica is a commonly occurring dinoflagellate species and a producer of diarrhetic shellfish poisons. Natural samples were collected from Trondheim fjord, Norway, to analyse nutrient (O, C, N, P) and toxin (dinophysitoxins [DXTs], okadaic acid [OA], pectenotoxins [PTXs]) content in D. norvegica cells. Nutrient and toxin analyses were also carried out on cells grown under nutrient-sufficient and nutrient-deficient conditions to determine how intracellular nutrient and toxin content varied under different nutrient availability conditions. Nutrient analyses were conducted using nuclear microprobe techniques that can accurately analyse single cells, and toxin analyses were carried out using liquid chromatography and mass spectroscopy. The intracellular carbon, nitrogen and phosphorus content in individual cells varied greatly, and intracellular C:N:P ratios showed that the cells were both N- and P-deficient when compared to the Redfield ratio. The ideal N:P ratio in the media for D. norvegica was found to be below the Redfield ratio, but intracellular ratios did not show a clear relationship with those in the media. N:P ratios of D. norvegica were higher than expected, which is likely due to their phagotrophy on zooplankon. The highest toxin values found were traces of PTX2, 24.72 pg PTX2SA cell(-1), 2.19 pg DTX1 cell(-1), and 1.01 pg OA cell(-1). However, we found no clear relationship between the content of intracellular nutrients and toxins.
Originalspråkengelska
Sidor (från-till)259-269
TidskriftAquatic Microbial Ecology
Volym75
Utgåva3
DOI
StatusPublished - 2015

Bibliografisk information

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Nuclear Physics (Faculty of Technology) (011013007), Aquatic Ecology (432112234)

Ämnesklassifikation (UKÄ)

  • Subatomär fysik
  • Ekologi

Fingeravtryck

Utforska forskningsämnen för ”Cellular nutrient content measured with the nuclear microprobe and toxins produced by Dinophysis norvegica (Dinophyceae) from the Trondheim fjord (Norway)”. Tillsammans bildar de ett unikt fingeravtryck.

Citera det här