Cellular Uptake of Cystatin C. Subcellular localisation and intracellular effects of a secreted cysteine protease inhibitor

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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Sammanfattning

Popular Abstract in English
The secreted cysteine protease inhibitor cystatin C has been thought to exert its functions extracellularly, as a defense against enzymes from leaking lysosomes or invading pathogens. In this thesis we demonstrate that regulation of intracellular proteases by cystatin C is possible, as a result of cellular uptake, and that the uptake can be modulated by molecular engineering of wild-type cystatin C. The uptake was seen in different epithelial and neuroblastoma cancer cell lines and was shown by various techniques, including flow cytometry, confocal microscopy, ELISA and Western blotting. Intracellular cysteine proteases involved in cancer-promoting processes may thus be controlled by cystatin C uptake.
Originalspråkengelska
KvalifikationDoktor
Tilldelande institution
  • Avdelningen för klinisk kemi och farmakologi
Handledare
  • Abrahamson, Magnus, handledare
  • Ekström, Ulf, handledare
Tilldelningsdatum2013 sep. 13
Förlag
ISBN (tryckt)978-91-87449-57-4
StatusPublished - 2013

Bibliografisk information

Defence details

Date: 2013-09-13
Time: 13:15
Place: Segerfalksalen, BMC, Sölvegatan 17, Lund

External reviewer(s)

Name: Theodorsson, Elvar
Title: [unknown]
Affiliation: Department of Clinical and Experimental Medicine, Division of Clinical Chemistry, Linköping University

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Ämnesklassifikation (UKÄ)

  • Farmakologi och toxikologi
  • Läkemedelskemi

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