Characterization of Mast Cells from Healthy and Varicose Human Saphenous Vein

Katrine T. Callesen, Sofia Mogren, Frida Berlin, Cecilia Andersson, Susanne Schmidt, Lotte Klitfod, Vanesa Esteban, Lars K. Poulsen, Bettina M. Jensen

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

Sammanfattning

Mast cells (MCs) are distributed in tissues throughout the body and are highly involved in many physiological and pathophysiological processes. The potential and involvement of different MC phenotypes are still not well understood. MCs are present in blood vessel walls, but their specific phenotypic features are unknown. We aimed at characterizing MCs from human saphenous veins for localization, mediator content, and receptor expression. This was done in MCs from both healthy and varicose human saphenous veins (hSV and vSV, respectively). For both vSV and hSV, we found that vein MCs are mainly present in the tunica adventitia (99% MCs in adventitia) and that the population consists of both MCT and MCTC phenotypes (vSV: 55% MCT, hSV: 64% MCT). The vein MCs contained high levels of histamine (vSV: 27 pg/MC, hSV: 55 pg/MC) and tryptase (vSV: 98 pg/MC, hSV: 111 pg/MC), indicating a strong potential for regulatory effects on blood vessels. The receptor expression of FcɛRI, MRGPRX2, PTAFR, C3aR, and C5aR was found, even though the percentage of positive cells differed between vSV and hSV MCs. We conclude that vein MCs from the blood vessel wall have a high potential to affect the tissue around them.

Originalspråkengelska
Artikelnummer1062
TidskriftBiomedicines
Volym10
Nummer5
DOI
StatusPublished - 2022 maj

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© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Ämnesklassifikation (UKÄ)

  • Hematologi

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