Chromosomal integration and expression of green fluorescent protein in Zymomonas mobilis

Osvaldo Delgado, MA Martinez, CM Abate, F Sineriz

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

7 Citeringar (SciVal)

Sammanfattning

An integrative vector was constructed to allow expression of heterologous proteins into the adhB locus of Zymomonas mobilis. As a reporter gene, the ORF of a bright variant of green fluorescent protein from Aequorea victoria (GFPuv) was fused to the adhB strong promoter from Z. mobilis by using a two-step PCR strategy. Z. mobilis recombinant strains that were stably marked by precise gene replacement at adhB locus with a single chromosomal copy of gfpuv. Protein expression was confirmed by fluorescence microscopy and measured by fluorescence spectroscopy, showing high expression levels (12 to 30 times higher than those obtained in E. coli) without affecting the host growth.
Originalspråkengelska
Sidor (från-till)1285-1290
TidskriftBiotechnology Letters
Volym24
Utgåva15
DOI
StatusPublished - 2002

Ämnesklassifikation (UKÄ)

  • Industriell bioteknik

Fingeravtryck

Utforska forskningsämnen för ”Chromosomal integration and expression of green fluorescent protein in Zymomonas mobilis”. Tillsammans bildar de ett unikt fingeravtryck.

Citera det här