Circulating Tumor Cell Kinetics and Morphology from the Liquid Biopsy Predict Disease Progression in Patients with Metastatic Colorectal Cancer Following Resection

Drahomir Kolenčík, Narayan Sachin, Jana-Aletta Thiele, Dillon McKinley, Anna Sandström Gerdtsson, Lisa Welter, Petr Hošek, Pavel Ostašov, Ondrej Vyčítal, Jan Brůha, Ondrej Fiala, Ondrej Šorejs, Vaclav Liska, Pavel Pitule, Peter Kuhn, Stephanie Shishido

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

Sammanfattning

The liquid biopsy has the potential to improve current clinical practice in oncology by
providing real-time personalized information about a patient’s disease status and response to treatment. In this study, we evaluated 161 peripheral blood (PB) samples that were collected around
surgical resection from 47 metastatic colorectal cancer (mCRC) patients using the High-Definition
Single Cell Assay (HDSCA) workflow. In conjunction with the standard circulating tumor cell (CTC)
enumeration, cellular morphology and kinetics between time-points of collection were considered in
the survival analysis. CTCs, CTC-Apoptotic, and CTC clusters were found to indicate poor survival
with an increase in cell count from pre-resection to post-resection. This study demonstrates that
CTC subcategorization based on morphological differences leads to nuanced results between the
subtypes, emphasizing the heterogeneity within the CTC classification. Furthermore, we show that
factoring in the time-point of each blood collection is critical, both for its static enumeration and for
the change in cell populations between draws. By integrating morphology and time-based analysis
alongside standard CTC enumeration, liquid biopsy platforms can provide greater insight into the
pathophysiology of mCRC by highlighting the complexity of the disease across a patient’s treatment.
Originalspråkengelska
TidskriftCancers
Volym14
Utgåva3
DOI
StatusPublished - 2022 jan. 27

Ämnesklassifikation (UKÄ)

  • Cancer och onkologi

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