Conformational change of giant DNA with added salt as revealed by single molecular observation

Naoko Makita, Magnus Ullner, Kenichi Yoshikawa

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36 Citeringar (SciVal)


The structural change of individual giant double-stranded T4 DNA (165.6 kilobase pairs) molecules as a function of salt concentration was investigated by single molecular observation with fluorescence microscopy in a wide range of concentration of NaCl, 1 x 10(-6)-3 M. The measured long-axis length was transformed into a persistence length and subjected to a power-law analysis, which showed that the electrostatic contribution to the persistence length is inversely proportional to the square root of the salt concentration; i.e., it has a linear dependence on the screening length. This is interpreted as the behavior of a flexible chain with electrostatic excluded-volume interactions. Although double-stranded DNA is locally stiff, it displays flexibility when the contour length is much longer than the persistence length, as is the case for T4 DNA.
Sidor (från-till)6200-6206
StatusPublished - 2006

Bibliografisk information

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Theoretical Chemistry (S) (011001039)

Ämnesklassifikation (UKÄ)

  • Teoretisk kemi


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