Sammanfattning
This protocol describes the design and use of CRISPRi-mediated transcriptional silencing in human iPSCs, for loss-of-function studies in brain development research. The protocol avoids single cell selection, thereby eliminating side effects of clonal expansion and sites of viral integration. We also describe a neural progenitor differentiation protocol and discuss the challenges of target-specific lentiviral silencing, efficient silencing levels, and off-target effects. For complete details on the use and execution of this protocol, please refer to Johansson et al. (2022).
Originalspråk | engelska |
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Artikelnummer | 101285 |
Tidskrift | STAR Protocols |
Volym | 3 |
Nummer | 2 |
DOI | |
Status | Published - 2022 juni 17 |
Ämnesklassifikation (UKÄ)
- Cell- och molekylärbiologi
- Neurovetenskaper