CRISPRi-mediated transcriptional silencing in iPSCs for the study of human brain development

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Sammanfattning

This protocol describes the design and use of CRISPRi-mediated transcriptional silencing in human iPSCs, for loss-of-function studies in brain development research. The protocol avoids single cell selection, thereby eliminating side effects of clonal expansion and sites of viral integration. We also describe a neural progenitor differentiation protocol and discuss the challenges of target-specific lentiviral silencing, efficient silencing levels, and off-target effects. For complete details on the use and execution of this protocol, please refer to Johansson et al. (2022).

Originalspråkengelska
Artikelnummer101285
TidskriftSTAR Protocols
Volym3
Nummer2
DOI
StatusPublished - 2022 juni 17

Ämnesklassifikation (UKÄ)

  • Cell- och molekylärbiologi
  • Neurovetenskaper

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