Crosstalk between mast cells and lung fibroblasts is modified by alveolar extracellular matrix and influences epithelial migration

Mariam Bagher, Oskar Rosmark, Linda Elowsson Rendin, Annika Nybom, Sebastian Wasserstrom, Catharina Müller, Xiao Hong Zhou, Göran Dellgren, Oskar Hallgren, Leif Bjermer, Anna Karin Larsson-Callerfelt, Gunilla Westergren-Thorsson

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

4 Citeringar (SciVal)

Sammanfattning

Mast cells play an important role in asthma, however, the interactions between mast cells, fibroblasts and epithelial cells in idiopathic pulmonary fibrosis (IPF) are less known. The objectives were to investigate the effect of mast cells on fibroblast activity and migration of epithelial cells. Lung fibroblasts from IPF patients and healthy individuals were co-cultured with LAD2 mast cells or stimulated with the proteases tryptase and chymase. Human lung fibroblasts and mast cells were cultured on cell culture plastic plates or decellularized human lung tissue (scaffolds) to create a more physiological milieu by providing an alveolar extracellular matrix. Released mediators were analyzed and evaluated for effects on epithelial cell migration. Tryptase increased vascular endothelial growth factor (VEGF) release from fibroblasts, whereas co-culture with mast cells increased IL-6 and hepatocyte growth factor (HGF). Culture in scaffolds increased the release of VEGF compared to culture on plastic. Migration of epithelial cells was reduced by IL-6, while HGF and conditioned media from scaffold cultures promoted migration. In conclusion, mast cells and tryptase increased fibroblast release of mediators that influenced epithelial migration. These data indicate a role of mast cells and tryptase in the interplay between fibroblasts, epithelial cells and the alveolar extracellular matrix in health and lung disease.

Originalspråkengelska
Artikelnummer506
Antal sidor16
TidskriftInternational Journal of Molecular Sciences
Volym22
Utgåva2
DOI
StatusPublished - 2021

Ämnesklassifikation (UKÄ)

  • Cell- och molekylärbiologi
  • Lungmedicin och allergi

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