TY - JOUR
T1 - Cryogenic probe technology enables multidimensional solid-state NMR of the stratum corneum without isotope labeling
AU - Perrone, Barbara
AU - Gunnarsson, Maria
AU - Bernin, Diana
AU - Sparr, Emma
AU - Topgaard, Daniel
PY - 2024/12
Y1 - 2024/12
N2 - Solid-state NMR has great potential for investigating molecular structure, dynamics, and organization of the stratum corneum, the outer 10–20 μm of the skin, but is hampered by the unfeasibility of isotope labelling as generally required to reach sufficient signal-to-noise ratio for the more informative multidimensional NMR techniques. In this preliminary study of pig stratum corneum at 35 °C and water-free conditions, we demonstrate that cryogenic probe technology offers sufficient signal boost to observe previously undetectable minor resonances that can be uniquely assigned to fluid cholesterol, ceramides, and triacylglycerols, as well as enables 1H–1H spin diffusion monitored by 2D 1H-13C HETCOR to estimate 1–100 nm distances between specific atomic sites on proteins and lipids. The new capabilities open up for future multidimensional solid-state NMR studies to answer long-standing questions about partitioning of additives, such as pharmaceutically active substances, between solid and liquid domains within the protein and lipid phases in the stratum corneum and the lipids of the sebum.
AB - Solid-state NMR has great potential for investigating molecular structure, dynamics, and organization of the stratum corneum, the outer 10–20 μm of the skin, but is hampered by the unfeasibility of isotope labelling as generally required to reach sufficient signal-to-noise ratio for the more informative multidimensional NMR techniques. In this preliminary study of pig stratum corneum at 35 °C and water-free conditions, we demonstrate that cryogenic probe technology offers sufficient signal boost to observe previously undetectable minor resonances that can be uniquely assigned to fluid cholesterol, ceramides, and triacylglycerols, as well as enables 1H–1H spin diffusion monitored by 2D 1H-13C HETCOR to estimate 1–100 nm distances between specific atomic sites on proteins and lipids. The new capabilities open up for future multidimensional solid-state NMR studies to answer long-standing questions about partitioning of additives, such as pharmaceutically active substances, between solid and liquid domains within the protein and lipid phases in the stratum corneum and the lipids of the sebum.
U2 - 10.1016/j.ssnmr.2024.101972
DO - 10.1016/j.ssnmr.2024.101972
M3 - Article
C2 - 39357420
AN - SCOPUS:85205314390
SN - 0926-2040
VL - 134
JO - Solid State Nuclear Magnetic Resonance
JF - Solid State Nuclear Magnetic Resonance
M1 - 101972
ER -