Decreased activities of apolipoprotein m promoter are associated with the susceptibility to coronary artery diseases.

Lu Zheng, Guanghua Luo, Jun Zhang, Qinfeng Mu, Yuanping Shi, Maria Berggren Söderlund, Peter Nilsson-Ehle, Xiaoying Zhang, Ning Xu

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

11 Citeringar (SciVal)
104 Nedladdningar (Pure)

Sammanfattning

The present study investigated the correlation among genetic polymorphisms of the proximal promoter region of apolipoprotein M (apoM) gene, the polymorphisms in relation to apoM expressions and the susceptibility to coronary artery diseases (CAD) in a Han Chinese population. Four common polymorphic sites, i.e., T-1628G, C-1065A, T-855C and T-778C, were confirmed, and a new deletion mutation C-724del was found, in 206 CAD patients and 209 non-CAD patients using direct DNA sequencing analyses. Occurrences of alleles T-1628G, T-855C and C-724del were significantly higher in CAD patients compared to non-CAD patients. Moreover we examined all these polymorphisms in relation to apoM expression by applying luciferase reporter assay. It demonstrated that constructs -855C and 724del showed obvious decreased luciferase activities, i.e., (0.93±0.15 vs. 2.11±0.15; P=0.012) and (1.13±0.25 vs. 2.11±0.15; P=0.009) respectively, which indicates these two polymorphisms could confer decreased apoM expressions. Meanwhile the occurrences of these two SNP were also significantly higher in the CAD patients than in non-CAD patients. It is therefore reasonable to speculate that down-regulated apoM expressions in relation to these polymorphisms may affect HDL and cholesterol metabolism in vivo and further influence the susceptibility to CAD, although the underlying mechanisms need further investigation.
Originalspråkengelska
Sidor (från-till)365-372
TidskriftInternational Journal of Medical Sciences
Volym11
Utgåva4
DOI
StatusPublished - 2014

Ämnesklassifikation (UKÄ)

  • Farmakologi och toxikologi
  • Läkemedelskemi

Fingeravtryck

Utforska forskningsämnen för ”Decreased activities of apolipoprotein m promoter are associated with the susceptibility to coronary artery diseases.”. Tillsammans bildar de ett unikt fingeravtryck.

Citera det här