TY - JOUR
T1 - Degradation of human secretory immunoglobulin A by Blastocystis
AU - Puthia, Manoj K
AU - Vaithilingam, Aparna
AU - Lu, Jia
AU - Tan, Kevin S W
PY - 2005/11
Y1 - 2005/11
N2 - Microbial immunoglobulin A (IgA) proteases cleave human secretory IgA, promoting the mucosal adhesion of pathogens. To investigate if the enteric protozoan Blastocystis degrades human secretory IgA, cell lysate and conditioned medium from two species were exposed to immunoglobulin A. Secretory IgA was cleaved by both cell lysate and conditioned medium with mainly cysteine proteinase activity in B. hominis B isolate and aspartic proteinase activity in B. ratii WR1 isolate. These findings suggest that Blastocystis proteases may play a role in parasite survival in vivo.
AB - Microbial immunoglobulin A (IgA) proteases cleave human secretory IgA, promoting the mucosal adhesion of pathogens. To investigate if the enteric protozoan Blastocystis degrades human secretory IgA, cell lysate and conditioned medium from two species were exposed to immunoglobulin A. Secretory IgA was cleaved by both cell lysate and conditioned medium with mainly cysteine proteinase activity in B. hominis B isolate and aspartic proteinase activity in B. ratii WR1 isolate. These findings suggest that Blastocystis proteases may play a role in parasite survival in vivo.
KW - Animals
KW - Aspartic Acid Endopeptidases/metabolism
KW - Blastocystis/enzymology
KW - Blastocystis hominis/enzymology
KW - Culture Media, Conditioned/metabolism
KW - Cysteine Endopeptidases/metabolism
KW - Humans
KW - Immunoglobulin A, Secretory/metabolism
KW - Rats
U2 - 10.1007/s00436-005-1461-0
DO - 10.1007/s00436-005-1461-0
M3 - Article
C2 - 16151742
VL - 97
SP - 386
EP - 389
JO - Parasitology Research
JF - Parasitology Research
SN - 1432-1955
IS - 5
ER -