Derivation of mouse embryonic stem cell lines from tyrosine hydroxylase reporter mice crossed with a human SNCA transgenic mouse model of Parkinson's disease

Margarita Chumarina; Carla Azevedo; Julie Bigarreau; Clémentine Vignal; Kwang Soo Kim; Jia-Yi Li; Laurent Roybon

doi:10.1016/j.scr.2016.12.026

Derivation of mouse embryonic stem cell lines from tyrosine hydroxylase reporter mice crossed with a human SNCA transgenic mouse model of Parkinson's disease

Margarita Chumarina, Carla Azevedo, Julie Bigarreau, Clémentine Vignal, Kwang Soo Kim, Jia-Yi Li, Laurent Roybon

Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

Sammanfattning

Mouse embryonic stem cell (mESC) lines were derived by crossing heterozygous transgenic (tg) mice expressing green fluorescent protein (GFP) under the control of the rat tyrosine hydroxylase (TH) promoter, with homozygous alpha-synuclein (aSYN) mice expressing human mutant SNCAA53T under the control of the mouse Prion promoter (MoPrP), or wildtype (WT) mice. The expression of GFP and human aSYN was validated by immunocytochemistry in midbrain neuron cultures upon differentiation of mESC lines using stromal cell-derived inducing activity. These mESC lines can help to study the impact of human aSYN expression in neurons and oligodendrocytes, and also trace GFP-expressing midbrain neurons.

Originalspråk	engelska
Sidor (från-till)	17-20
Antal sidor	4
Tidskrift	Stem Cell Research
Volym	19
DOI	https://doi.org/10.1016/j.scr.2016.12.026
Status	Published - 2017 mars 1

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2 Doktorsavhandling (sammanläggning)

Oligodendroglial (dys)function in alpha-synucleinopathies
Azevedo, C., 2020, Lund: Lund University, Faculty of Medicine. 119 s.
Forskningsoutput: Avhandling › Doktorsavhandling (sammanläggning)

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Stem cell-based models to study cellular alterations in Parkinson’s Disease
Chumarina, M., 2020, Lund: Lund University, Faculty of Medicine. 84 s.
Forskningsoutput: Avhandling › Doktorsavhandling (sammanläggning)

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title = "Derivation of mouse embryonic stem cell lines from tyrosine hydroxylase reporter mice crossed with a human SNCA transgenic mouse model of Parkinson's disease",

abstract = "Mouse embryonic stem cell (mESC) lines were derived by crossing heterozygous transgenic (tg) mice expressing green fluorescent protein (GFP) under the control of the rat tyrosine hydroxylase (TH) promoter, with homozygous alpha-synuclein (aSYN) mice expressing human mutant SNCAA53T under the control of the mouse Prion promoter (MoPrP), or wildtype (WT) mice. The expression of GFP and human aSYN was validated by immunocytochemistry in midbrain neuron cultures upon differentiation of mESC lines using stromal cell-derived inducing activity. These mESC lines can help to study the impact of human aSYN expression in neurons and oligodendrocytes, and also trace GFP-expressing midbrain neurons.",

author = "Margarita Chumarina and Carla Azevedo and Julie Bigarreau and Cl{\'e}mentine Vignal and Kim, {Kwang Soo} and Jia-Yi Li and Laurent Roybon",

year = "2017",

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AU - Chumarina, Margarita

AU - Azevedo, Carla

AU - Bigarreau, Julie

AU - Vignal, Clémentine

AU - Kim, Kwang Soo

AU - Li, Jia-Yi

AU - Roybon, Laurent

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Mouse embryonic stem cell (mESC) lines were derived by crossing heterozygous transgenic (tg) mice expressing green fluorescent protein (GFP) under the control of the rat tyrosine hydroxylase (TH) promoter, with homozygous alpha-synuclein (aSYN) mice expressing human mutant SNCAA53T under the control of the mouse Prion promoter (MoPrP), or wildtype (WT) mice. The expression of GFP and human aSYN was validated by immunocytochemistry in midbrain neuron cultures upon differentiation of mESC lines using stromal cell-derived inducing activity. These mESC lines can help to study the impact of human aSYN expression in neurons and oligodendrocytes, and also trace GFP-expressing midbrain neurons.

AB - Mouse embryonic stem cell (mESC) lines were derived by crossing heterozygous transgenic (tg) mice expressing green fluorescent protein (GFP) under the control of the rat tyrosine hydroxylase (TH) promoter, with homozygous alpha-synuclein (aSYN) mice expressing human mutant SNCAA53T under the control of the mouse Prion promoter (MoPrP), or wildtype (WT) mice. The expression of GFP and human aSYN was validated by immunocytochemistry in midbrain neuron cultures upon differentiation of mESC lines using stromal cell-derived inducing activity. These mESC lines can help to study the impact of human aSYN expression in neurons and oligodendrocytes, and also trace GFP-expressing midbrain neurons.

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Derivation of mouse embryonic stem cell lines from tyrosine hydroxylase reporter mice crossed with a human SNCA transgenic mouse model of Parkinson's disease

Sammanfattning

FN:s Globala mål

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Forskningsoutput

Oligodendroglial (dys)function in alpha-synucleinopathies

Stem cell-based models to study cellular alterations in Parkinson’s Disease

Citera det här