Development of an amperometric biosensor based on acetylcholine esterase covalently bound to a new support material

M Khayyami, M T Pérez Pita, N Peña Garcia, Gillis Johansson, Bengt Danielsson, Per-Olof Larsson

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

Sammanfattning

A new type of amperometric biosensor based on immobilised acetylcholine esterase was designed and constructed. The enzyme was immobilised on a flow-through working electrode, which was prepared from reticulated vitreous carbon (RVC) or from a composite material consisting of RVC and superporous agarose. The sensor was operated in FIA mode using acetylthiocholine as a substrate. The sensor responded to inhibitors such as paraoxon—10−9 mol was detected by the sensor in a non-optimised configuration. The practical lifetime of the sensor was at least 1 month.
Originalspråkengelska
Sidor (från-till)557-563
TidskriftTalanta
Volym45
Nummer3
DOI
StatusPublished - 1998

Ämnesklassifikation (UKÄ)

  • Kompositmaterial och -teknik
  • Analytisk kemi

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