Development of an automated flow-based spectrophotometric immunoassay for continuous detection of zearalenone

Jongjit Jantra, Kinga Zór, Melanie Sanders, Sarah De Saeger, Martin Hedström, Bo Mattiasson

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

3 Citeringar (SciVal)

Sammanfattning

Considering the widespread contaminations of food products with mycotoxins, it is important to develop, robust, time- and cost-effective detection methods. We developed and optimized an immunoassay using a continuous flow system for the detection of zearalenone (ZEN). The assay was performed in a flow mode using an automated sequential injection system. Time for an assay cycle was 18 Min. Under optimal conditions, the limit for quantification for ZEN was 0.40 µg L−1 with a linear dependency between concentration and signal amplitude between 0.10 and 10 µg L−1. The assay proved to be robust and reliable with 13% relative standard deviation between measurements. By dissociating the antigen–antibody complex using a regeneration solution, we showed 50 times reusability of the immobilized antibodies without affecting the antigen-binding properties.

Originalspråkengelska
Sidor (från-till)375-382
Antal sidor8
TidskriftBiotechnology and Applied Biochemistry
Volym67
Utgåva3
DOI
StatusPublished - 2020 maj 1

Ämnesklassifikation (UKÄ)

  • Biokemi och molekylärbiologi

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