Development of microformat imaging microplate and membrane immunoenzyme assays of the herbicide atrazine

O D Hendrickson, Ioana Wärnmark, R Sitdikov, A V Zherdev, B B Dzantiev, Bengt Danielsson

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

1 Citering (SciVal)

Sammanfattning

A microformat imaging technique in combination with registration of chemiluminescence by a charge-coupled device, CCD camera, were applied for the detection of the herbicide atrazine by two immunoenzyme assay formats - microplate and membrane assays. For the microplate (ELISA) case the detection limit was 95 pg mL(-1), giving a 9.5-fold enhancement in comparison with a traditional colorimetric assay. The membrane dot blot assay allowed detection of up to 40 pg mL(-1) of atrazine (4.5-fold enhancement). Due to the application of polyelectrolyte carriers for rapid reactants separation the membrane assay may be realized in 15 min. CCD-based registration of signals assures a high reproducibility of measurements; the variation coefficient is in the range 1-7%. The effectiveness of the assays developed for controlling triazine herbicides in mineral and tap water has been demonstrated.
Originalspråkengelska
Sidor (från-till)905-915
TidskriftInternational Journal of Environmental Analytical Chemistry
Volym85
Utgåva12-13
DOI
StatusPublished - 2005

Ämnesklassifikation (UKÄ)

  • Biokemi och molekylärbiologi

Fingeravtryck

Utforska forskningsämnen för ”Development of microformat imaging microplate and membrane immunoenzyme assays of the herbicide atrazine”. Tillsammans bildar de ett unikt fingeravtryck.

Citera det här