Distinct phenotypes of platelet, monocyte, and neutrophil activation occur during the acute and convalescent phase of COVID-19

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

55 Nedladdningar (Pure)


SARS-CoV-2 has spread rapidly worldwide, causing the COVID-19 pandemic. Platelet activation and platelet-leukocyte complex formation are proposed to contribute to disease progression. Here, we report platelet and leukocyte activation during acute and convalescent COVID-19 in patients recruited between May-July 2020. Blood samples were analyzed by flow cytometry and ELISA using paired comparison between inclusion (day 0) and 28 days later. The majority of patients were mildly or moderately ill with significantly higher cytokine levels (IL-6 and IL-10) on day 0 as compared with day 28. Platelet activation and granule release were significantly higher on day 0 compared with day 28, as determined by ADP- or thrombin-induced surface CD62P expression, baseline released CD62P, and thrombin-induced platelet-monocyte complex formation. Monocyte activation and procoagulant status at baseline and post activation were heterogeneous but generally lower on day 0 compared with day 28. Baseline and thrombin- or fMLF-induced neutrophil activation and procoagulant status were significantly lower on day 0 compared with day 28. We demonstrate that during the acute phase of COVID-19 compared with the convalescent phase, platelets are more responsive while neutrophils are less responsive. COVID-19 is associated with thromboembolic events where platelet activation and interaction with leukocytes may play an important role.

Sidor (från-till)1092–1102
StatusPublished - 2021

Bibliografisk information

Funding Information:
This work was supported by grants by the Swedish Research Council (Vetenskapsrådet, VR) under grants 2018-05795 (O.S.) and 2017-01779 (J.W.S.); Avtal om Läkarutbildning och Forskning under grant 8439 (J.W.S.); and Avtal om Landstingsanknuten Forskning under grant 2018-0253 (M.R.). We thank the hospital staff for assistance with blood sampling. We thank Professor Mattias Collin (Lund University) for promptly coordinating and establishing a working environment for COVID-19 studies. We thank Fredrik Kahn (Lund University) for important advice regarding the study.

Publisher Copyright:
© 2021 The Author(s). Published with license by Taylor & Francis Group, LLC.

Copyright 2021 Elsevier B.V., All rights reserved.

Ämnesklassifikation (UKÄ)

  • Infektionsmedicin
  • Mikrobiologi inom det medicinska området
  • Hematologi


Utforska forskningsämnen för ”Distinct phenotypes of platelet, monocyte, and neutrophil activation occur during the acute and convalescent phase of COVID-19”. Tillsammans bildar de ett unikt fingeravtryck.

Citera det här