Downstream Processing Using Membrane Technology

Anna Persson

Downstream Processing Using Membrane Technology

Anna Persson

Avdelningen för kemiteknik

Forskningsoutput: Avhandling › Doktorsavhandling (monografi)

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Popular Abstract in Swedish
Membranteknik är en separationsteknik där stora partiklar skiljs av från mindre partiklar m.h.a. ett membranfilter. I min forskning har jag tittat på hur membranfiltrering kan användas inom bioteknikindustrin. Jag har tittat på hur mjölksyra kan renas upp från en lösning av jäst "vetemjölsvälling" samt hur första steget i uppreningen av protein A från en industriellt producerad fermenteringslösning på bästa sätt kan designas med membranteknik. Tekniker som jag använt är mikrofiltrering, ultrafiltrering och elektrodialys. Mikrofiltrereing har jag använt för att separera mikroorganismer från lösningen. Med ultrafiltrering har jag avlägsnat stora molekyler så som proteiner från lösningen. M.h.a. elektrodialys har jag separerat laddade molekyler från oladdade molekyler samt omvandlat saltet natrium-laktat (NaLa) till mjölksyra (HLa). Mjölksyra kan användas för produktion av plast att användas till förpackningsmaterial och suturer (de trådar som operationsläkaren syr ihop dig med efter operationen). Fördelen med mjölksyraplast är att den är lättnedbrytbar samt att nedbrytningsprodukterna inte är farliga för miljön. Protein A används inom bioteknikindustrin för tillverkning av materiel till andra separationsmetoder som t.ex. kromatografi.

Originalspråk	engelska
Kvalifikation	Doktor
Tilldelande institution	Avdelningen för kemiteknik
Handledare	Zacchi, Guido, handledare
Tilldelningsdatum	2001 sep. 14
Förlag	Department of Chemical Engineering, Lund University
ISBN (tryckt)	91-628-4875-5
Status	Published - 2001

Bibliografisk information

Defence details

Date: 2001-09-14
Time: 13:15
Place: Room B at The Center for Chemistry and Chemical Engineering (KC)

External reviewer(s)

Name: Bowen, Richard
Title: Prof
Affiliation: Uiniversity of Wales, Swansea

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title = "Downstream Processing Using Membrane Technology",

abstract = "Membrane filtration is a very gentle separation technique and can be used at low temperatures. This makes it suitable for liquids that contain micro-organisms and/or are heat sensitive, which is often the case in liquids processed in the field of biotechnology. However, one drawback is that these liquids are often very complex, which is a disadvantage when using membrane technology as it makes the process difficult to optimise regarding both process parameters and cleaning. This thesis deals with different aspects of membrane filtration and how membranes can be applied in different stages of the downstream processing. The work presented in this thesis covers downstream processing in the production of lactic acid from fermented wheat starch. In this process microfiltration and ultrafiltration are used to remove micro-organisms and proteins from the broth. Finally, water-splitting electrodialysis is used to convert the sodium lactate produced during the fermentation to lactic acid and sodium hydroxide. The use of sub-critical flux when separating micro-organisms from lactic-acid-containing fermentation broth has also been studied. Part of the work deals with the retention of proteins in microfiltration membranes and how this is affected by pH, ion strength and membrane material. Two different proteins, bovine serum albumin and protein A have been studied.",

keywords = "Kemiteknik och kemisk teknologi, Biochemical technology, Chemical technology and engineering, Protein A, Bovine serum albumin, Lactic acid, Electrodialysis, Ultrafiltration, Microfiltration, Downstream processing, Membrane, Biokemisk teknik",

author = "Anna Persson",

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N1 - Defence details Date: 2001-09-14 Time: 13:15 Place: Room B at The Center for Chemistry and Chemical Engineering (KC) External reviewer(s) Name: Bowen, Richard Title: Prof Affiliation: Uiniversity of Wales, Swansea ---

PY - 2001

Y1 - 2001

N2 - Membrane filtration is a very gentle separation technique and can be used at low temperatures. This makes it suitable for liquids that contain micro-organisms and/or are heat sensitive, which is often the case in liquids processed in the field of biotechnology. However, one drawback is that these liquids are often very complex, which is a disadvantage when using membrane technology as it makes the process difficult to optimise regarding both process parameters and cleaning. This thesis deals with different aspects of membrane filtration and how membranes can be applied in different stages of the downstream processing. The work presented in this thesis covers downstream processing in the production of lactic acid from fermented wheat starch. In this process microfiltration and ultrafiltration are used to remove micro-organisms and proteins from the broth. Finally, water-splitting electrodialysis is used to convert the sodium lactate produced during the fermentation to lactic acid and sodium hydroxide. The use of sub-critical flux when separating micro-organisms from lactic-acid-containing fermentation broth has also been studied. Part of the work deals with the retention of proteins in microfiltration membranes and how this is affected by pH, ion strength and membrane material. Two different proteins, bovine serum albumin and protein A have been studied.

AB - Membrane filtration is a very gentle separation technique and can be used at low temperatures. This makes it suitable for liquids that contain micro-organisms and/or are heat sensitive, which is often the case in liquids processed in the field of biotechnology. However, one drawback is that these liquids are often very complex, which is a disadvantage when using membrane technology as it makes the process difficult to optimise regarding both process parameters and cleaning. This thesis deals with different aspects of membrane filtration and how membranes can be applied in different stages of the downstream processing. The work presented in this thesis covers downstream processing in the production of lactic acid from fermented wheat starch. In this process microfiltration and ultrafiltration are used to remove micro-organisms and proteins from the broth. Finally, water-splitting electrodialysis is used to convert the sodium lactate produced during the fermentation to lactic acid and sodium hydroxide. The use of sub-critical flux when separating micro-organisms from lactic-acid-containing fermentation broth has also been studied. Part of the work deals with the retention of proteins in microfiltration membranes and how this is affected by pH, ion strength and membrane material. Two different proteins, bovine serum albumin and protein A have been studied.

KW - Kemiteknik och kemisk teknologi

KW - Biochemical technology

KW - Chemical technology and engineering

KW - Protein A

KW - Bovine serum albumin

KW - Lactic acid

KW - Electrodialysis

KW - Ultrafiltration

KW - Microfiltration

KW - Downstream processing

KW - Membrane

KW - Biokemisk teknik

M3 - Doctoral Thesis (monograph)

SN - 91-628-4875-5

T3 - Department of Chemical Engineering 1, Lund University, Sweden

PB - Department of Chemical Engineering, Lund University

ER -

Downstream Processing Using Membrane Technology

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