Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome: A SYSDIET sub-study

Lena Leder, Marjukka Kolehmainen, Ingunn Narverud, Ingrid Dahlman, Mari C W Myhrstad, Vanessa D. De Mello, Jussi Paananen, Carsten Carlberg, Ursula Schwab, Karl Heinz Herzig, Lieselotte Cloetens, Matilda Ulmius, Janne Hukkanen, Markku J. Savolainen, Fredrik Rosqvist, Kjeld Hermansen, Lars O. Dragsted, Gunnarsdottir Ingibjörg Gunnarsdottir, Inga Thorsdottir, Ulf RisérusBjörn Åkesson, Magne Thoresen, Peter Arner, Kaisa S. Poutanen, Matti Uusitupa, Kirsten B. Holven, Stine M. Ulven

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Background: Diet has a great impact on the risk of developing features of metabolic syndrome (MetS), type 2 diabetes mellitus (T2DM), and cardiovascular diseases (CVD). We evaluated whether a long-term healthy Nordic diet (ND) can modify the expression of inflammation and lipid metabolism-related genes in peripheral blood mononuclear cells (PBMCs) during a 2-h oral glucose tolerance test (OGTT) in individuals with MetS. Methods: A Nordic multicenter randomized dietary study included subjects (n = 213) with MetS, randomized to a ND group or a control diet (CD) group applying an isocaloric study protocol. In this sub-study, we included subjects (n = 89) from three Nordic centers: Kuopio (n =26), Lund (n = 30), and Oulu (n = 33) with a maximum weight change of ±4 kg, high-sensitivity C-reactive protein concentration ≤10 mg L-1, and baseline body mass index -2. PBMCs were isolated, and the mRNA gene expression analysis was measured by quantitative real-time polymerase chain reaction (qPCR). We analyzed the mRNA expression changes of 44 genes before and after a 2hOGTT at the beginning and the end of the intervention. Results: The healthy ND significantly down-regulated the expression of toll-like receptor 4 (TLR4), interleukin 18 (IL18), and thrombospondin receptor (CD36) mRNA transcripts and significantly up-regulated the expression of peroxisome proliferator-activated receptor delta (PPARD) mRNA transcript after the 2hOGTT compared to the CD. Conclusions: A healthy ND is able to modify the gene expression in PBMCs after a 2hOGTT. However, more studies are needed to clarify the biological and clinical relevance of these findings.

Originalspråkengelska
TidskriftGenes & Nutrition
Volym11
Nummer1
DOI
StatusPublished - 2016 mars 17

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