Electrical communication of cytochrome enriched Escherichia coli JM109 cells with graphite electrodes

Sergey Alferov, Vasile Coman, Tobias Gustavsson, Anatoly Reshetilov, Claes von Wachenfeldt, Cecilia Hägerhäll, Lo Gorton

Forskningsoutput: Kapitel i bok/rapport/Conference proceedingKonferenspaper i proceedingPeer review

27 Citeringar (SciVal)

Sammanfattning

In the present study three different strains of Escherichia coli (JM 109 - a native "wild type" strain, JM 109/pBSD 1300 - a strain overproducing the membrane anchor domain of Bacillus subtilis succinate-quinone reductase, SQR, a protein that contains two transmembraneously arranged heme groups and JM109/pLUV 1900 - a strain overproducing cytochrome c(550) from B. subtilis, a protein where the cytochrome domain is anchored to the membrane with a transmembrane helix) were immobilised on the surface of a spectrographic graphite electrode and tested for electrical communication using mediators. Such compounds as ferricyanide, 2,6-dichlorophenolindophenol (DCPIP) and ubiquinone (Q(0)) were used as soluble mediators and two flexible osmium redox polymers; poly(1-vinylimidazole)(12)-[Os-(4,4'-dimethyl-2,2'-di'pyridyl)(2)Cl-2](2 +/+) (osmium redox polymer I) and poly(vinylpyridine)-[Os-(N,N'-methylated-2,2'-biimidazole)(3)](2+/3+) (osmium redox polymer II) were co-immobilised with the bacterial cells onto the electrode surface. The effects of applied potential, buffer pH and different substrates were compared for the different combinations bacterial strains - mediators. Through the introduction of the cytochromes in the bacterial membrane it was established that it had great effect on the ability of the bacterial cells to effectively communicate with artificial mediators. The introduction of the transmembraneously arranged heme groups of B. subtilis made it possible for this strain to communicate with the Os-polymers, whereas the introduction of the cytochrome c(550) had an effect especially increasing ability of Q(0) to act as an efficient e(-) acceptor. (C) 2009 Elsevier Ltd. All rights reserved.
Originalspråkengelska
Titel på värdpublikationElectrochimica Acta
FörlagElsevier
Sidor4979-4984
Volym54
DOI
StatusPublished - 2009
Evenemang59th Annual Meeting of the International-Society-of-Electrochemistry - Seville, Spanien
Varaktighet: 2008 sep. 72008 sep. 12

Publikationsserier

Namn
Nummer22
Volym54
ISSN (tryckt)1873-3859
ISSN (elektroniskt)0013-4686

Konferens

Konferens59th Annual Meeting of the International-Society-of-Electrochemistry
Land/TerritoriumSpanien
OrtSeville
Period2008/09/072008/09/12

Bibliografisk information

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Biochemistry and Structural Biology (S) (000006142), Molecular Cell Biology (432112241)

Ämnesklassifikation (UKÄ)

  • Biologiska vetenskaper

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