Evaluation of simple enzyme kinetics by response surface modelling

Mats Andersson; Patrick Adlercreutz

doi:10.1023/A:1008994613645

Evaluation of simple enzyme kinetics by response surface modelling

Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

Sammanfattning

The activity of a horse liver alcohol dehydrogenase catalysed reduction of cyclohexanone was investigated by using a central composite circumscribed design in which two parameters (pH and cyclohexanone concentration) were varied. By log transformation of the substrate concentration an adequate model could be obtained from which reliable kinetic constants and pH profiles were determined.

Originalspråk	engelska
Sidor (från-till)	903-907
Antal sidor	5
Tidskrift	Biotechnology Techniques
Volym	13
Nummer	12
DOI	https://doi.org/10.1023/A:1008994613645
Status	Published - 1999 dec. 1

Ämnesklassifikation (UKÄ)

Biokatalys och enzymteknik

Tillgång till dokument

10.1023/A:1008994613645

Andra filer och länkar

Link to publication in Scopus

Citera det här

@article{0fabffbc23254b00b2bb67c96b13e65b,

title = "Evaluation of simple enzyme kinetics by response surface modelling",

abstract = "The activity of a horse liver alcohol dehydrogenase catalysed reduction of cyclohexanone was investigated by using a central composite circumscribed design in which two parameters (pH and cyclohexanone concentration) were varied. By log transformation of the substrate concentration an adequate model could be obtained from which reliable kinetic constants and pH profiles were determined.",

keywords = "Alcohol dehydrogenase, Cyclohexanone, Enzyme kinetics, Response surface modelling",

author = "Mats Andersson and Patrick Adlercreutz",

year = "1999",

month = dec,

day = "1",

doi = "10.1023/A:1008994613645",

language = "English",

volume = "13",

pages = "903--907",

journal = "Biotechnology Techniques",

issn = "0951-208X",

publisher = "Springer Science and Business Media B.V.",

number = "12",

}

TY - JOUR

T1 - Evaluation of simple enzyme kinetics by response surface modelling

AU - Andersson, Mats

AU - Adlercreutz, Patrick

PY - 1999/12/1

Y1 - 1999/12/1

N2 - The activity of a horse liver alcohol dehydrogenase catalysed reduction of cyclohexanone was investigated by using a central composite circumscribed design in which two parameters (pH and cyclohexanone concentration) were varied. By log transformation of the substrate concentration an adequate model could be obtained from which reliable kinetic constants and pH profiles were determined.

AB - The activity of a horse liver alcohol dehydrogenase catalysed reduction of cyclohexanone was investigated by using a central composite circumscribed design in which two parameters (pH and cyclohexanone concentration) were varied. By log transformation of the substrate concentration an adequate model could be obtained from which reliable kinetic constants and pH profiles were determined.

KW - Alcohol dehydrogenase

KW - Cyclohexanone

KW - Enzyme kinetics

KW - Response surface modelling

UR - http://www.scopus.com/inward/record.url?scp=0033380112&partnerID=8YFLogxK

U2 - 10.1023/A:1008994613645

DO - 10.1023/A:1008994613645

M3 - Article

AN - SCOPUS:0033380112

SN - 0951-208X

VL - 13

SP - 903

EP - 907

JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 12

ER -

Evaluation of simple enzyme kinetics by response surface modelling

Sammanfattning

Ämnesklassifikation (UKÄ)

Tillgång till dokument

Andra filer och länkar

Fingeravtryck

Citera det här