Ex Vivo Working Porcine Heart Model

Harry Pigot; Kristian Soltesz; Stig Steen

doi:10.1007/978-1-0716-3846-0_7

Ex Vivo Working Porcine Heart Model

Harry Pigot, Kristian Soltesz, Stig Steen

Forskningsoutput: Kapitel i bok/rapport/Conference proceeding › Kapitel samlingsverk › Forskning

Sammanfattning

Ex vivo working porcine heart models allow for the study of a heart’s function and physiology outside the living organism. These models are particularly useful due to the anatomical and physiological similarities between porcine and human hearts, providing an experimental platform to investigate cardiac disease or assess donor heart viability for transplantation. This chapter presents an in-depth discussion of the model’s components, including the perfusate, preload, and afterload. We explore the challenges of emulating cardiac afterload and present a historical perspective on afterload modeling, discussing various methodologies and their respective limitations. An actively controlled afterload device is introduced to enhance the model’s ability to rapidly adjust pressure in the large arteries, thereby providing a more accurate and dynamic experimental model. Finally, we provide a comprehensive experimental protocol for the ex vivo working porcine heart model.

Originalspråk	engelska
Titel på värdpublikation	Experimental Models of Cardiovascular Diseases
Undertitel på värdpublikation	Methods and Protocols
Redaktörer	Kiyotake Ishikawa
Utgivningsort	New York, NY
Förlag	Humana Press
Kapitel	7
Sidor	87-107
Antal sidor	20
Utgåva	2
ISBN (elektroniskt)	978-1-0716-3846-0
ISBN (tryckt)	978-1-0716-3848-4, 978-1-0716-3845-3
DOI	https://doi.org/10.1007/978-1-0716-3846-0_7
Status	Published - 2024 apr. 28

Publikationsserier

Namn	Methods in Molecular Biology
Förlag	Springer Protocols
Volym	2803
ISSN (tryckt)	1064-3745
ISSN (elektroniskt)	1940-6029

FN:s Globala mål

Denna forskningsoutput relaterar till följande Globala mål

SDG 3 – God hälsa och välbefinnande

Ämnesklassifikation (UKÄ)

Medicinsk instrumentering (Här ingår: Mätteknik)
Medicinsk bioteknologi
Kardiologi och kardiovaskulära sjukdomar
Reglerteknik

Tillgång till dokument

10.1007/978-1-0716-3846-0_7Licens: CC BY

Andra filer och länkar

Link to publication in Scopus

5 Artikel i vetenskaplig tidskrift
1 Konferenspaper i proceeding
1 Doktorsavhandling (sammanläggning)

Afterload system design for functional donor heart assessment
Pigot, H., 2024 mars, Sweden: Department of Automatic Control, Lund University. 137 s.
Forskningsoutput: Avhandling › Doktorsavhandling (sammanläggning)

Öppen tillgång
Fil
Estimating cardiac mechanical efficiency in a porcine ex situ working heart model
Pigot, H., Steen, S. & Soltesz, K., 2024, I: IFAC Proceedings Volumes (IFAC-PapersOnline). s. 55-60 6 s.
Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

Öppen tillgång
Fil
47 Nedladdningar (Pure)
Actively controlled cardiac afterload
Pigot, H., Wahlquist, Y. & Soltesz, K., 2023, I: IFAC Proceedings Volumes (IFAC-PapersOnline). 56, 2, s. 6484-6489
Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

Öppen tillgång
Fil
78 Nedladdningar (Pure)

Functional ex vivo heart evaluation
Steen, E. (Forskarstuderande), Soltesz, K. (PI), Nilsson, J. (PI) & Pigot, H. (Forskare)
Familjen Hjelms stiftelse för medicinsk forskning, Mats Paulssons stiftelse
2023/12/01 → …
Projekt: Forskning

Citera det här

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title = "Ex Vivo Working Porcine Heart Model",

abstract = "Ex vivo working porcine heart models allow for the study of a heart{\textquoteright}s function and physiology outside the living organism. These models are particularly useful due to the anatomical and physiological similarities between porcine and human hearts, providing an experimental platform to investigate cardiac disease or assess donor heart viability for transplantation. This chapter presents an in-depth discussion of the model{\textquoteright}s components, including the perfusate, preload, and afterload. We explore the challenges of emulating cardiac afterload and present a historical perspective on afterload modeling, discussing various methodologies and their respective limitations. An actively controlled afterload device is introduced to enhance the model{\textquoteright}s ability to rapidly adjust pressure in the large arteries, thereby providing a more accurate and dynamic experimental model. Finally, we provide a comprehensive experimental protocol for the ex vivo working porcine heart model.",

keywords = "Working porcine heart, Isolated heart preparation, Functional heart evaluation, cardiac afterload, cyber-physiological systems, functional heart assessment, ex vivo heart perfusion, ex situ heart perfusion, ex vivo heart evaluation, heart transplantation",

author = "Harry Pigot and Kristian Soltesz and Stig Steen",

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doi = "10.1007/978-1-0716-3846-0\_7",

language = "English",

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series = "Methods in Molecular Biology",

publisher = "Humana Press",

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editor = "Kiyotake Ishikawa",

booktitle = "Experimental Models of Cardiovascular Diseases",

address = "United States",

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Ex Vivo Working Porcine Heart Model. / Pigot, Harry ; Soltesz, Kristian ; Steen, Stig.
Experimental Models of Cardiovascular Diseases: Methods and Protocols. red. / Kiyotake Ishikawa. 2. uppl. New York, NY: Humana Press, 2024. s. 87-107 (Methods in Molecular Biology; Vol. 2803).

Forskningsoutput: Kapitel i bok/rapport/Conference proceeding › Kapitel samlingsverk › Forskning

TY - CHAP

T1 - Ex Vivo Working Porcine Heart Model

AU - Pigot, Harry

AU - Soltesz, Kristian

AU - Steen, Stig

PY - 2024/4/28

Y1 - 2024/4/28

N2 - Ex vivo working porcine heart models allow for the study of a heart’s function and physiology outside the living organism. These models are particularly useful due to the anatomical and physiological similarities between porcine and human hearts, providing an experimental platform to investigate cardiac disease or assess donor heart viability for transplantation. This chapter presents an in-depth discussion of the model’s components, including the perfusate, preload, and afterload. We explore the challenges of emulating cardiac afterload and present a historical perspective on afterload modeling, discussing various methodologies and their respective limitations. An actively controlled afterload device is introduced to enhance the model’s ability to rapidly adjust pressure in the large arteries, thereby providing a more accurate and dynamic experimental model. Finally, we provide a comprehensive experimental protocol for the ex vivo working porcine heart model.

AB - Ex vivo working porcine heart models allow for the study of a heart’s function and physiology outside the living organism. These models are particularly useful due to the anatomical and physiological similarities between porcine and human hearts, providing an experimental platform to investigate cardiac disease or assess donor heart viability for transplantation. This chapter presents an in-depth discussion of the model’s components, including the perfusate, preload, and afterload. We explore the challenges of emulating cardiac afterload and present a historical perspective on afterload modeling, discussing various methodologies and their respective limitations. An actively controlled afterload device is introduced to enhance the model’s ability to rapidly adjust pressure in the large arteries, thereby providing a more accurate and dynamic experimental model. Finally, we provide a comprehensive experimental protocol for the ex vivo working porcine heart model.

KW - Working porcine heart

KW - Isolated heart preparation

KW - Functional heart evaluation

KW - cardiac afterload

KW - cyber-physiological systems

KW - functional heart assessment

KW - ex vivo heart perfusion

KW - ex situ heart perfusion

KW - ex vivo heart evaluation

KW - heart transplantation

UR - https://www.scopus.com/pages/publications/85191639979

U2 - 10.1007/978-1-0716-3846-0_7

DO - 10.1007/978-1-0716-3846-0_7

M3 - Book chapter

C2 - 38676887

SN - 978-1-0716-3848-4

SN - 978-1-0716-3845-3

T3 - Methods in Molecular Biology

SP - 87

EP - 107

BT - Experimental Models of Cardiovascular Diseases

A2 - Ishikawa, Kiyotake

PB - Humana Press

CY - New York, NY

ER -

Ex Vivo Working Porcine Heart Model

Sammanfattning

Publikationsserier

FN:s Globala mål

Ämnesklassifikation (UKÄ)

Tillgång till dokument

Andra filer och länkar

Fingeravtryck

Afterload system design for functional donor heart assessment

Estimating cardiac mechanical efficiency in a porcine ex situ working heart model

Actively controlled cardiac afterload

Functional ex vivo heart evaluation

Citera det här

Ex Vivo Working Porcine Heart Model

Sammanfattning

Publikationsserier

FN:s Globala mål

Ämnesklassifikation (UKÄ)

Tillgång till dokument

Andra filer och länkar

Fingeravtryck

Forskningsoutput

Afterload system design for functional donor heart assessment

Estimating cardiac mechanical efficiency in a porcine ex situ working heart model

Actively controlled cardiac afterload

Projekt

Functional ex vivo heart evaluation

Citera det här