Expression and purification of rat glucose transporter 1 in Pichia pastoris

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Sammanfattning

Large amounts of pure and homogenous protein are a prerequisite for several biochemical and biophysical analyses, and in particular if aiming at resolving the three-dimensional protein structure. Here we describe the production of the rat glucose transporter 1 (GLUT1), a membrane protein facilitating the transport of glucose in cells. The protein is recombinantly expressed in the yeast Pichia pastoris. It is easily maintained and large-scale protein production in shaker flasks, as commonly performed in academic research laboratories, results in relatively high yields of membrane protein. The purification protocol describes all steps needed to obtain a pure and homogenous GLUT1 protein solution, including cell growth, membrane isolation, and chromatographic purification methods.

Originalspråkengelska
Titel på värdpublikationMethods in Molecular Biology
FörlagHumana Press
Antal sidor13
Volym1713
DOI
StatusPublished - 2018

Publikationsserier

NamnMethods in Molecular Biology
Volym1713
ISSN (tryckt)1064-3745

Ämnesklassifikation (UKÄ)

  • Cell- och molekylärbiologi

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