Fibulin-1 is a ligand for the C-type lectin domains of aggrecan and versican

Anders Aspberg, S Adam, G Kostka, R Timpl, Dick Heinegård

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review


The aggregating proteoglycans (aggrecan, versican, neurocan, and brevican) are important components of many extracellular matrices. Their N-terminal globular domain binds to hyaluronan, but the function of their C-terminal region containing a C-type lectin domain is less clear. We now report that a 90-kDa protein copurifies with recombinant lectin domains from aggrecan and versican, but not from the brain-specific neurocan and brevican. Amino acid sequencing of tryptic peptides from this protein identified it as fibulin-1. This extracellular matrix glycoprotein is strongly expressed in tissues where versican is expressed (blood vessels, skin, and developing heart), and also expressed in developing cartilage and bone. It is thus likely to interact with these proteoglycans in vivo. Surface plasmon resonance measurements confirmed that aggrecan and versican lectin domains bind fibulin-1, whereas brevican and neurocan do not. As expected for a C-type lectin, the interactions with fibulin-1 are Ca2+-dependent, with KD values in the low nanomolar range. Using various deletion mutants, the binding site for aggrecan and versican lectin domains was mapped to the epidermal growth factor-like repeats in domain II of fibulin-1. No difference in affinity was found for deglycosylated fibulin-1, indicating that the proteoglycan C-type lectin domains bind to the protein part of fibulin-1.
Sidor (från-till)20444-20449
TidskriftJournal of Biological Chemistry
StatusPublished - 1999

Bibliografisk information

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Connective Tissue Biology (013230151)

Ämnesklassifikation (UKÄ)

  • Reumatologi och inflammation


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