Filter-exchange PGSE NMR determination of cell membrane permeability.

Ingrid Åslund, Agnieszka Nowacka, Markus Nilsson, Daniel Topgaard

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

73 Citeringar (SciVal)

Sammanfattning

A new PGSE NMR sequence is introduced for measuring diffusive transport across the plasma membrane of living cells. A "diffusion filter" and a variable mixing time precedes a standard PGSE block for diffusion encoding of the NMR signal. The filter is a PGSE block optimized for selectively removing the magnetization of the extracellular water. With increasing mixing time the intra- and extracellular components approach their equilibrium fractional populations. The rate of exchange can be measured using only a few minutes of instrument time. Water exchange over the plasma membrane of starved yeast cells is studied in the temperature range +5 to +32 degrees C.
Originalspråkengelska
Sidor (från-till)291-295
TidskriftJournal of Magnetic Resonance
Volym200
DOI
StatusPublished - 2009

Ämnesklassifikation (UKÄ)

  • Fysikalisk kemi

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