@inbook{bd52798123eb420a93f712174cd2e3df,
title = "Flow Cytometry Assay of Plasmodium Falciparum-Specific B-Cell Proportions",
abstract = "Detection of P. falciparum-specific subpopulations of B-cells is important for studies of immunity in malaria. This protocol relies on the photostability and protein loading capacity of carboxylated quantum dots to detect a broad range of different P. falciparum-specific B-cells. Infected red blood cell ghosts, obtained by permeabilization of infected cells with Streptolysin O, are coupled with carboxyl quantum dots using N-ethyl-N-dimethylaminopropyl-carbodiimide condensation. Immunophenotyping of P. falciparum-specific B-cells is performed by flow cytometry using Fc-receptor block, quantum dot-infected red blood cell ghost conjugates, and fluorochrome-conjugated anti-human CD19 mouse monoclonal antibodies.",
keywords = "B-cells, Flow cytometry, Ghost infected red blood cells, Malaria, Plasmodium falciparum, Quantum dots",
author = "Allan Lugaajju and Tijani, {Muyideen Kolapo} and Persson, {Kristina Eva Maria}",
year = "2022",
doi = "10.1007/978-1-0716-2189-9_51",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "681--688",
booktitle = "Methods in Molecular Biology",
address = "United States",
}
