Glucose transport activity measured in giant vesicles

Jesper S. Hansen, Karin Lindkvist-Petersson

Forskningsoutput: Kapitel i bok/rapport/Conference proceedingKapitel samlingsverkPeer review

Sammanfattning

Incorporation of membrane proteins and internal reporter systems directly into giant vesicles, during their formation from a hydrogel surface, has emerged as a promising new concept in membrane protein characterization. Here, we provide the detailed protocol for a glucose transporter activity assay based on giant vesicles containing a fluorescent enzyme-linked reporter system internally. This assay is applicable for the functional analysis of a variety of hexose-transporting proteins. We furthermore believe that it can aid in the development of drugs targeting hexose transporters.

Originalspråkengelska
Titel på värdpublikationMethods in Molecular Biology
FörlagHumana Press
Sidor77-91
Antal sidor15
Volym1713
DOI
StatusPublished - 2018

Publikationsserier

NamnMethods in Molecular Biology
Volym1713
ISSN (tryckt)1064-3745

Ämnesklassifikation (UKÄ)

  • Cell- och molekylärbiologi

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