Glucuronosylated and linear xylooligosaccharides from Quinoa stalks xylan as potential prebiotic source for growth of Bifidobacterium adolescentis and Weissella cibaria

Quinoa stalks glucuronoarabinoxylan (QSGAX) has been extracted by
alkali and further utilized for production of two-types of
xylooligosaccharides (XOs): i) enzymatically produced
glucuronosylated-XOs (GXOs), and ii) a dilute acid produced mixture of
non-substituted/substituted XOs of different degree of polymerization
(DP). The respective mixtures were then separately evaluated as prebiotics by analysis of their consumption by two phylogenetically different potential probiotic bacterial strains (Bifidobacterium adolescentis ATCC15703 and Weissella cibaria strain 92), from which release of short chain fatty acids (SCFA) was also monitored. The GXOs mixture was produced using a
glucuronosyl-requiring family 30 glycoside hydrolase (GH) Bacteroides ovatus (BoXyn30A), while the XOs mixture was produced by a 30 min acid treatment of QSGAX with H₂SO₄ [24.5 g/L] at 90 °C. B. adolescentis
consumed both GXOs and XOs (DP 2–6), in both cases releasing acetate,
lactate, propionate, formate and butyrate as metabolic products. W. cibaria
only consumed XOs (DP 2–4), releasing acetate, lactate and minor
amounts of butyrate. This is the first study reporting the ability of
GXOs consumption by B. adolescentis and shows the potential of GXOs to selectively stimulate B. adolescentis, while XOs stimulated both types of potential probiotics (B. adolescentis ATCC15703 and W. cibaria strain 92).