Histidine protonation states are key in the LigI catalytic reaction mechanism

Lina Zhao, Dibyendu Mondal, Weifeng Li, Yuguang Mu, Philipp Kaldis

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

Sammanfattning

Lignin is one of the world's most abundant organic polymers, and 2-pyrone-4,6-dicarboxylate lactonase (LigI) catalyzes the hydrolysis of 2-pyrone-4,6-dicarboxylate (PDC) in the degradation of lignin. The pH has profound effects on enzyme catalysis and therefore we studied this in the context of LigI. We found that changes of the pH mostly affects surface residues, while the residues at the active site are more subject to changes of the surrounding microenvironment. In accordance with this, a high pH facilitates the deprotonation of the substrate. Detailed free energy calculations by the empirical valence bond (EVB) approach revealed that the overall hydrolysis reaction is more likely when the three active site histidines (His31, His33 and His180) are protonated at the &ip.eop; site, however, protonation at the δ site may be favored during specific steps of the reaction. Our studies have uncovered the determinant role of the protonation state of the active site residues His31, His33 and His180 in the hydrolysis of PDC.

Originalspråkengelska
Sidor (från-till)123-130
TidskriftProteins
Volym90
Nummer1
Tidigt onlinedatum2021 juli 27
DOI
StatusPublished - 2022

Ämnesklassifikation (UKÄ)

  • Biokemi och molekylärbiologi

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