Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes

Christa Broholm; Anders Henrik Olsson; Alexander Perfilyev; Linn Gillberg; Ninna Schiøler Hansen; Ashfaq Ali; Brynjulf Mortensen; Charlotte Ling; Allan Vaag

doi:10.2217/epi-2016-0077

Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes

Christa Broholm, Anders Henrik Olsson, Alexander Perfilyev, Linn Gillberg, Ninna Schiøler Hansen, Ashfaq Ali, Brynjulf Mortensen, Charlotte Ling, Allan Vaag

Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

Sammanfattning

Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.

Originalspråk	engelska
Sidor (från-till)	1601-1617
Antal sidor	17
Tidskrift	Epigenomics
Volym	8
Nummer	12
DOI	https://doi.org/10.2217/epi-2016-0077
Status	Published - 2016 dec. 1

Ämnesklassifikation (UKÄ)

Endokrinologi och diabetes
Medicinsk genetik och genomik (Här ingår: Genterapi)

FN:s Globala mål

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10.2217/epi-2016-0077

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National Bioinformatics Infrastructure Sweden, NBIS
Ahrén, D. (Manager), Ringnér, M. (Manager), Levander, F. (Manager), Manoharan, L. (Manager), Oskolkov, N. (Manager), Vasquez, L. J. A. (Manager), Pyl, P. T. (Manager), Agarwal, P. (Manager), Li, Y. (Manager), Volpe, M. (Manager) & Kozjek, K. (Manager)
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title = "Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes",

abstract = "Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.",

keywords = "adipogenesis, adiponectin, DNA methylation, epigenetics, stem cells",

author = "Christa Broholm and Olsson, {Anders Henrik} and Alexander Perfilyev and Linn Gillberg and Hansen, {Ninna Schi{\o}ler} and Ashfaq Ali and Brynjulf Mortensen and Charlotte Ling and Allan Vaag",

year = "2016",

month = dec,

day = "1",

doi = "10.2217/epi-2016-0077",

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journal = "Epigenomics",

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T1 - Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes

AU - Broholm, Christa

AU - Olsson, Anders Henrik

AU - Perfilyev, Alexander

AU - Gillberg, Linn

AU - Hansen, Ninna Schiøler

AU - Ali, Ashfaq

AU - Mortensen, Brynjulf

AU - Ling, Charlotte

AU - Vaag, Allan

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.

AB - Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.

KW - adipogenesis

KW - adiponectin

KW - DNA methylation

KW - epigenetics

KW - stem cells

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Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes

Sammanfattning

Ämnesklassifikation (UKÄ)

FN:s Globala mål

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Utrustning

National Bioinformatics Infrastructure Sweden, NBIS

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