Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes

Christa Broholm; Anders Henrik Olsson; Alexander Perfilyev; Linn Gillberg; Ninna Schiøler Hansen; Ashfaq Ali; Brynjulf Mortensen; Charlotte Ling; Allan Vaag

doi:10.2217/epi-2016-0077

Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes

Christa Broholm, Anders Henrik Olsson, Alexander Perfilyev, Linn Gillberg, Ninna Schiøler Hansen, Ashfaq Ali, Brynjulf Mortensen, Charlotte Ling, Allan Vaag

Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

Sammanfattning

Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.

Originalspråk	engelska
Sidor (från-till)	1601-1617
Antal sidor	17
Tidskrift	Epigenomics
Volym	8
Nummer	12
DOI	https://doi.org/10.2217/epi-2016-0077
Status	Published - 2016 dec. 1

Ämnesklassifikation (UKÄ)

Endokrinologi och diabetes
Medicinsk genetik

Tillgång till dokument

10.2217/epi-2016-0077

Andra filer och länkar

Link to publication in Scopus

National Bioinformatics Infrastructure Sweden, NBIS
Dag Ahrén (Manager), Markus Ringnér (Manager), Fredrik Levander (Manager), Ashfaq Ali (Manager), Lokeshwaran Manoharan (Manager), Nikolay Oskolkov (Manager), Louella Judy Antonio Vasquez (Manager), Paul Theodor Pyl (Manager), Juliana Assis Geraldo (Manager) & Prasoon Agarwal (Manager)
Biologiska institutionen
Infrastruktur

Citera det här

@article{655811c8e44942148138959205118aeb,

title = "Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes",

abstract = "Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.",

keywords = "adipogenesis, adiponectin, DNA methylation, epigenetics, stem cells",

author = "Christa Broholm and Olsson, {Anders Henrik} and Alexander Perfilyev and Linn Gillberg and Hansen, {Ninna Schi{\o}ler} and Ashfaq Ali and Brynjulf Mortensen and Charlotte Ling and Allan Vaag",

year = "2016",

month = dec,

day = "1",

doi = "10.2217/epi-2016-0077",

language = "English",

volume = "8",

pages = "1601--1617",

journal = "Epigenomics",

issn = "1750-192X",

publisher = "Future Medicine Ltd.",

number = "12",

}

TY - JOUR

T1 - Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes

AU - Broholm, Christa

AU - Olsson, Anders Henrik

AU - Perfilyev, Alexander

AU - Gillberg, Linn

AU - Hansen, Ninna Schiøler

AU - Ali, Ashfaq

AU - Mortensen, Brynjulf

AU - Ling, Charlotte

AU - Vaag, Allan

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.

AB - Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.

KW - adipogenesis

KW - adiponectin

KW - DNA methylation

KW - epigenetics

KW - stem cells

UR - http://www.scopus.com/inward/record.url?scp=85002944734&partnerID=8YFLogxK

U2 - 10.2217/epi-2016-0077

DO - 10.2217/epi-2016-0077

M3 - Article

C2 - 27854126

AN - SCOPUS:85002944734

VL - 8

SP - 1601

EP - 1617

JO - Epigenomics

JF - Epigenomics

SN - 1750-192X

IS - 12

ER -

Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes

Sammanfattning

Ämnesklassifikation (UKÄ)

Tillgång till dokument

Andra filer och länkar

Fingeravtryck

Utrustning

National Bioinformatics Infrastructure Sweden, NBIS

Citera det här