Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes

Christa Broholm, Anders Henrik Olsson, Alexander Perfilyev, Linn Gillberg, Ninna Schiøler Hansen, Ashfaq Ali, Brynjulf Mortensen, Charlotte Ling, Allan Vaag

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskriftPeer review

Sammanfattning

Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.

Originalspråkengelska
Sidor (från-till)1601-1617
Antal sidor17
TidskriftEpigenomics
Volym8
Nummer12
DOI
StatusPublished - 2016 dec. 1

Ämnesklassifikation (UKÄ)

  • Endokrinologi och diabetes
  • Medicinsk genetik

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