Human papillomaviruses in skin cancer and cervical cancer

Kristin Andersson

Human papillomaviruses in skin cancer and cervical cancer

Kristin Andersson

Forskningsoutput: Avhandling › Doktorsavhandling (sammanläggning)

466 Nedladdningar (Pure)

Sammanfattning

Popular Abstract in Swedish
Papillomvirus (PV) är små virus som förmodligen infekterar alla däggdjur
samt fåglar. De papillomvirus som infekterar människa kallas humant papillomvirus
(HPV) och kan infektera antingen huden eller slemhinnor.
Att livmoderhalscancer orsakas av bestående infektion med HPV har varit
känt länge. Man har länge också misstänkt att HPV-infektioner i huden kan
orsaka icke-melanom hudcancer (NMSC) men orsakssambandet är inte bevisat.
I gruppen NMSC ingår i huvudsak diagnoserna skivepitelcancer (SCC) och
basalcellscancer (BCC). Vi har undersökt risken att utveckla NMSC om man
har en HPV-infektion i huden tillsammans med andra kända riskfaktorer för
hudcancer och om detektion av antikroppar mot HPV som infekterar huden
sammanfaller med förekomst av HPV-DNA i huden. Vi har även tittat på hur
sexuellt överförbara HPV-infektioner samverkar med andra faktorer, så som andra
infektioner och rökning, i utvecklingen av livmoderhalscancer.
Två av studierna om NMSC är designade som sjukhusbaserade fall-kontrollstudier
(individer som redan har en sjukdom jämförs med individer utan sjukdomen),
där vävnad från tumör och frisk hud analyserats för förekomst av HPVDNA
och serumprov testats för förekomst av antikroppar mot 14 olika HPVtyper.
En tredje NMSC-studie och en studie om livmoderhalscancer är båda
designade som prospektiva (framåtblickande) fall-kontroll-studier där biobanker
länkats till cancerregister för att identifiera individer med sjukdomen som lämnat
prov till biobanken. Inom varje biobank har kontroller sedan valts efter
matchning mot fallen (faktorer så som kön, ålder och tidpunkt för provtagning)
och serumprov från både fall och kontroller samlats in och analyserats. Från
fallen med livmoderhalscancer har även tumörvävnad testats för HPV-DNA.
Sammanfattningsvis fann vi att infektion i huden med HPV från genus beta
species 2 innebar en ökad risk att utveckla SCC i huden samt att förhöjd exponering
av huden för solljus var en riskfaktor för att få en HPV-infektion. För
livmoderhalscancer fann vi att om DNA-test och antikroppstest var positivt för samma HPV-typ ökade risken att utveckla livmoderhalscancer jämfört med om
man bara testats positiv för antikroppar eller om HPV-typerna inte överensstämde.
Att ha varit infekterad med Chlamydia trachomatis var också kopplat
till livmoderhalscancer och bidrar troligen till risken.

Originalspråk	engelska
Kvalifikation	Doktor
Tilldelande institution	Klinisk mikrobiologi, Malmö
Handledare	Dillner, Joakim, handledare Forslund, Ola, handledare Arnheim, Lisen, handledare, Extern person
Tilldelningsdatum	2010 apr. 22
Förlag	Department of Medical Microbiology, Lund University
ISBN (tryckt)	978-91-86443-55-9
Status	Published - 2010

Bibliografisk information

Defence details

Date: 2010-04-22
Time: 09:00
Place: lecturing hall pathology department, Malmö

External reviewer(s)

Name: favre, michel
Title: professor
Affiliation: Pasteur Institute, Paris, France

---

Ämnesklassifikation (UKÄ)

Mikrobiologi inom det medicinska området

FN:s Globala mål

Denna forskningsoutput relaterar till följande Globala mål

Tillgång till dokument

Kappa_Kristin_Andersson

2 Artikel i vetenskaplig tidskrift

Seroreactivity to Cutaneous Human Papillomaviruses among Patients with Nonmelanoma Skin Cancer or Benign Skin Lesions.
Andersson, K., Waterboer, T., Kirnbauer, R., Slupetzky, K., Iftner, T., de Villiers, E-M., Forslund, O., Pawlita, M. & Dillner, J., 2008, I: Cancer Epidemiology Biomarkers & Prevention. 17, 1, s. 189-195
Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

Öppen tillgång
Cutaneous human papillomaviruses found in sun-exposed skin: Beta-papillomavirus species 2 predominates in squamous cell carcinoma
Forslund, O., Iftner, T., Andersson, K., Lindelof, B., Hradil, E., Nordin, P., Stenquist, B., Kirnbauer, R., Dillner, J. & de Villiers, E-M., 2007, I: Journal of Infectious Diseases. 196, 6, s. 876-883
Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift › Peer review

Citera det här

@phdthesis{dcdc1f349b8e4cc991c36fa0ca51e33b,

title = "Human papillomaviruses in skin cancer and cervical cancer",

abstract = "The causal relationship between persistent genital infections with human papillomavirus (HPV) and development of cervical cancer is well established. In contrast, the significance of infections with cutaneous HPV for development of non-melanoma skin cancer (NMSC) is not well understood. We have evaluated whether seropositivity to cutaneous HPV is a marker for cutaneous HPV infection and used high throughput HPV serology to investigate the risk for developing NMSC in relation to seropositivity for cutaneous HPV infection and PCR techniques to investigate the risk for NMSC in relation to presence of HPV DNA in the skin. We have also investigated how different sexually transmitted infections interact with HPV in the aetiology of cervical cancer. Two of our NMSC studies were hospital-based case-control studies where biopsies from skin tumours and healthy skin were analysed for presence of HPV DNA and serum samples for presence of antibodies to 14 different HPV types. The third NMSC study and the cervical cancer study were designed as prospective biobank-based case-control studies where biobanks were linked to cancer registries for identification of cancers that have occurred after donation of a serum sample. For patients with cervix cancer also formalin-fixed paraffin embedded tumour tissue was retrieved and tested for HPV DNA. In the skin cancer studies, we found that both DNA and seropositivity to HPV of genus beta species 2 associated with an increased risk for development of squamous cell carcinoma (SCC) of the skin and that sun-exposure is a risk factor for cutaneous HPV infection. In the cervical cancer study we found in addition to the exposure to the oncogenic HPV type that is found in the cancer tissue, that history of Chlamydia trachomatis stood out among the different sexually transmitted infections as being associated with increased risk for cervical cancer, suggesting that it may acts as a co-factor to HPV in cervical carcinogenesis.",

keywords = "Human papillomavirus, serology co-factors, non-melanoma skin cancer",

author = "Kristin Andersson",

note = "Defence details Date: 2010-04-22 Time: 09:00 Place: lecturing hall pathology department, Malm{\"o} External reviewer(s) Name: favre, michel Title: professor Affiliation: Pasteur Institute, Paris, France ---",

year = "2010",

language = "English",

isbn = "978-91-86443-55-9",

series = "Lund University Faculty of Medicine Doctoral Dissertation Series ",

publisher = "Department of Medical Microbiology, Lund University",

type = "Doctoral Thesis (compilation)",

school = "Clinical Microbiology, Malm{\"o}",

}

TY - THES

T1 - Human papillomaviruses in skin cancer and cervical cancer

AU - Andersson, Kristin

N1 - Defence details Date: 2010-04-22 Time: 09:00 Place: lecturing hall pathology department, Malmö External reviewer(s) Name: favre, michel Title: professor Affiliation: Pasteur Institute, Paris, France ---

PY - 2010

Y1 - 2010

N2 - The causal relationship between persistent genital infections with human papillomavirus (HPV) and development of cervical cancer is well established. In contrast, the significance of infections with cutaneous HPV for development of non-melanoma skin cancer (NMSC) is not well understood. We have evaluated whether seropositivity to cutaneous HPV is a marker for cutaneous HPV infection and used high throughput HPV serology to investigate the risk for developing NMSC in relation to seropositivity for cutaneous HPV infection and PCR techniques to investigate the risk for NMSC in relation to presence of HPV DNA in the skin. We have also investigated how different sexually transmitted infections interact with HPV in the aetiology of cervical cancer. Two of our NMSC studies were hospital-based case-control studies where biopsies from skin tumours and healthy skin were analysed for presence of HPV DNA and serum samples for presence of antibodies to 14 different HPV types. The third NMSC study and the cervical cancer study were designed as prospective biobank-based case-control studies where biobanks were linked to cancer registries for identification of cancers that have occurred after donation of a serum sample. For patients with cervix cancer also formalin-fixed paraffin embedded tumour tissue was retrieved and tested for HPV DNA. In the skin cancer studies, we found that both DNA and seropositivity to HPV of genus beta species 2 associated with an increased risk for development of squamous cell carcinoma (SCC) of the skin and that sun-exposure is a risk factor for cutaneous HPV infection. In the cervical cancer study we found in addition to the exposure to the oncogenic HPV type that is found in the cancer tissue, that history of Chlamydia trachomatis stood out among the different sexually transmitted infections as being associated with increased risk for cervical cancer, suggesting that it may acts as a co-factor to HPV in cervical carcinogenesis.

AB - The causal relationship between persistent genital infections with human papillomavirus (HPV) and development of cervical cancer is well established. In contrast, the significance of infections with cutaneous HPV for development of non-melanoma skin cancer (NMSC) is not well understood. We have evaluated whether seropositivity to cutaneous HPV is a marker for cutaneous HPV infection and used high throughput HPV serology to investigate the risk for developing NMSC in relation to seropositivity for cutaneous HPV infection and PCR techniques to investigate the risk for NMSC in relation to presence of HPV DNA in the skin. We have also investigated how different sexually transmitted infections interact with HPV in the aetiology of cervical cancer. Two of our NMSC studies were hospital-based case-control studies where biopsies from skin tumours and healthy skin were analysed for presence of HPV DNA and serum samples for presence of antibodies to 14 different HPV types. The third NMSC study and the cervical cancer study were designed as prospective biobank-based case-control studies where biobanks were linked to cancer registries for identification of cancers that have occurred after donation of a serum sample. For patients with cervix cancer also formalin-fixed paraffin embedded tumour tissue was retrieved and tested for HPV DNA. In the skin cancer studies, we found that both DNA and seropositivity to HPV of genus beta species 2 associated with an increased risk for development of squamous cell carcinoma (SCC) of the skin and that sun-exposure is a risk factor for cutaneous HPV infection. In the cervical cancer study we found in addition to the exposure to the oncogenic HPV type that is found in the cancer tissue, that history of Chlamydia trachomatis stood out among the different sexually transmitted infections as being associated with increased risk for cervical cancer, suggesting that it may acts as a co-factor to HPV in cervical carcinogenesis.

KW - Human papillomavirus

KW - serology co-factors

KW - non-melanoma skin cancer

M3 - Doctoral Thesis (compilation)

SN - 978-91-86443-55-9

T3 - Lund University Faculty of Medicine Doctoral Dissertation Series

PB - Department of Medical Microbiology, Lund University

ER -

Human papillomaviruses in skin cancer and cervical cancer

Sammanfattning

Bibliografisk information

Ämnesklassifikation (UKÄ)

FN:s Globala mål

Tillgång till dokument

Fingeravtryck

Forskningsoutput

Seroreactivity to Cutaneous Human Papillomaviruses among Patients with Nonmelanoma Skin Cancer or Benign Skin Lesions.

Cutaneous human papillomaviruses found in sun-exposed skin: Beta-papillomavirus species 2 predominates in squamous cell carcinoma

Citera det här